BMRB Entry 25340

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25340

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Title:
Backbone assignments for human Cyclophilin D with saturating concentrations of the model peptide GSFGPDLRAGD
Deposition date:
2014-11-13
Original release date:
2014-12-16
Authors:
Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan
Citation:

Citation: Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan. "Structure and Dynamics of GeoCyp: A Thermophilic Cyclophilin with a Novel Substrate Binding Mechanism That Functions Efficiently at Low Temperatures"  Biochemistry 54, 3207-3217 (2015).
PubMed: 25923019

Assembly members:

Assembly members:
CypD, polymer, 166 residues, 17787.2 Da.
GSFGPDLRAGD, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CypD: GSGNPLVYLDVDANGKPLGR VVLELKADVVPKTAENFRAL CTGEKGFGYKGSTFHRVIPS FMCQAGDFTNHNGTGGKSIY GSRFPDENFTLKHVGPGVLS MANAGPNTNGSQFFICTIKT DWLDGKHVVFGHVKEGMDVV KKIESFGSKSGRTSKKIVIT DCGQLS
GSFGPDLRAGD: GSFGPDLRAGD

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts150
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CypD1
2GSFGPDLRAGD2

Entities:

Entity 1, CypD 166 residues - 17787.2 Da.

Residues 43-207 of human Cyclophilin D with one additional N-terminal residue, Gly-, remaining after removal of affinity tag

1   GLYSERGLYASNPROLEUVALTYRLEUASP
2   VALASPALAASNGLYLYSPROLEUGLYARG
3   VALVALLEUGLULEULYSALAASPVALVAL
4   PROLYSTHRALAGLUASNPHEARGALALEU
5   CYSTHRGLYGLULYSGLYPHEGLYTYRLYS
6   GLYSERTHRPHEHISARGVALILEPROSER
7   PHEMETCYSGLNALAGLYASPPHETHRASN
8   HISASNGLYTHRGLYGLYLYSSERILETYR
9   GLYSERARGPHEPROASPGLUASNPHETHR
10   LEULYSHISVALGLYPROGLYVALLEUSER
11   METALAASNALAGLYPROASNTHRASNGLY
12   SERGLNPHEPHEILECYSTHRILELYSTHR
13   ASPTRPLEUASPGLYLYSHISVALVALPHE
14   GLYHISVALLYSGLUGLYMETASPVALVAL
15   LYSLYSILEGLUSERPHEGLYSERLYSSER
16   GLYARGTHRSERLYSLYSILEVALILETHR
17   ASPCYSGLYGLNLEUSER

Entity 2, GSFGPDLRAGD 11 residues - Formula weight is not available

1   GLYSERPHEGLYPROASPLEUARGALAGLY
2   ASP

Related Database Links:

PDB 2BIT 2BIU 2Z6W 3QYU 3R49 3R4G 3R54 3R56 3R57 3R59 3RCF 3RCG 3RCI 3RCK 3RCL 3RD9 3RDA 3RDB 3RDC 4J58 4J59 4J5A 4J5B 4J5C 4J5D 4J5E 4O8H 4O8I 4XNC 4ZSC 4ZSD
DBJ BAG35623 BAG59266 BAI47316
GB AAA58434 AAH05020 AAI10300 ACE87134 ADQ32373
REF NP_001253053 NP_005720 XP_001929518 XP_002820831 XP_002922456
SP P30405
AlphaFold P30405

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks