BMRB Entry 25330

Name: Backbone Assignment of the heterodimer HUAB from E. coli at 293K
Published: 2015-05-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25330

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignment of the heterodimer HUAB from E. coli at 293K

Deposition date:

2014-11-12

Original release date:

2015-05-19

Authors:

Le Meur, Remy; Castaing, Bertrand; Landon, Celine; Loth, Karine

Citation:

Citation: Le Meur, Remy; Castaing, Bertrand; Landon, Celine; Loth, Karine. "Backbone assignment of the three dimers of HU from Escherichia coli at 293 K: EcHUalpha2, EcHUbeta2 2 and EcHUalphabeta2" Biomol. NMR Assignments 9, 359-363 (2015).
PubMed: 25924603

Assembly members:

Assembly members:
HUAB-A, polymer, 90 residues, Formula weight is not available
HUAB-B, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E.coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJES-hupA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HUAB-A: MNKTQLIDVIAEKAELSKTQ AKAALESTLAAITESLKEGD AVQLVGFGTFKVNHRAERTG RNPQTGKEIKIAAANVPAFV SGKALKDAVK
HUAB-B: MNKSQLIDKIAAGADISKAA AGRALDAIIASVTESLKEGD DVALVGFGTFAVKERAARTG RNPQTGKEITIAAAKVPSFR AGKALKDAVN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	501
15N chemical shifts	168
1H chemical shifts	168

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HUAB_1	1
2	HUAB_2	2

Entities:

Entity 1, HUAB_1 90 residues - Formula weight is not available

1	MET	ASN	LYS	THR	GLN	LEU	ILE	ASP	VAL	ILE
2	ALA	GLU	LYS	ALA	GLU	LEU	SER	LYS	THR	GLN
3	ALA	LYS	ALA	ALA	LEU	GLU	SER	THR	LEU	ALA
4	ALA	ILE	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ALA	VAL	GLN	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	LYS	VAL	ASN	HIS	ARG	ALA	GLU	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	LYS
8	ILE	ALA	ALA	ALA	ASN	VAL	PRO	ALA	PHE	VAL
9	SER	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	LYS

Entity 2, HUAB_2 90 residues - Formula weight is not available

1	MET	ASN	LYS	SER	GLN	LEU	ILE	ASP	LYS	ILE
2	ALA	ALA	GLY	ALA	ASP	ILE	SER	LYS	ALA	ALA
3	ALA	GLY	ARG	ALA	LEU	ASP	ALA	ILE	ILE	ALA
4	SER	VAL	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ASP	VAL	ALA	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	ALA	VAL	LYS	GLU	ARG	ALA	ALA	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	THR
8	ILE	ALA	ALA	ALA	LYS	VAL	PRO	SER	PHE	ARG
9	ALA	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	ASN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 25330

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	ASN	LYS	THR	GLN	LEU	ILE	ASP	VAL	ILE
2	ALA	GLU	LYS	ALA	GLU	LEU	SER	LYS	THR	GLN
3	ALA	LYS	ALA	ALA	LEU	GLU	SER	THR	LEU	ALA
4	ALA	ILE	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ALA	VAL	GLN	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	LYS	VAL	ASN	HIS	ARG	ALA	GLU	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	LYS
8	ILE	ALA	ALA	ALA	ASN	VAL	PRO	ALA	PHE	VAL
9	SER	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	LYS

1	MET	ASN	LYS	SER	GLN	LEU	ILE	ASP	LYS	ILE
2	ALA	ALA	GLY	ALA	ASP	ILE	SER	LYS	ALA	ALA
3	ALA	GLY	ARG	ALA	LEU	ASP	ALA	ILE	ILE	ALA
4	SER	VAL	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ASP	VAL	ALA	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	ALA	VAL	LYS	GLU	ARG	ALA	ALA	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	THR
8	ILE	ALA	ALA	ALA	LYS	VAL	PRO	SER	PHE	ARG
9	ALA	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	ASN

1	MET	ASN	LYS	THR	GLN	LEU	ILE	ASP	VAL	ILE
2	ALA	GLU	LYS	ALA	GLU	LEU	SER	LYS	THR	GLN
3	ALA	LYS	ALA	ALA	LEU	GLU	SER	THR	LEU	ALA
4	ALA	ILE	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ALA	VAL	GLN	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	LYS	VAL	ASN	HIS	ARG	ALA	GLU	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	LYS
8	ILE	ALA	ALA	ALA	ASN	VAL	PRO	ALA	PHE	VAL
9	SER	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	LYS

1	MET	ASN	LYS	SER	GLN	LEU	ILE	ASP	LYS	ILE
2	ALA	ALA	GLY	ALA	ASP	ILE	SER	LYS	ALA	ALA
3	ALA	GLY	ARG	ALA	LEU	ASP	ALA	ILE	ILE	ALA
4	SER	VAL	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ASP	VAL	ALA	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	ALA	VAL	LYS	GLU	ARG	ALA	ALA	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	THR
8	ILE	ALA	ALA	ALA	LYS	VAL	PRO	SER	PHE	ARG
9	ALA	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	ASN

1	MET	ASN	LYS	THR	GLN	LEU	ILE	ASP	VAL	ILE
2	ALA	GLU	LYS	ALA	GLU	LEU	SER	LYS	THR	GLN
3	ALA	LYS	ALA	ALA	LEU	GLU	SER	THR	LEU	ALA
4	ALA	ILE	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ALA	VAL	GLN	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	LYS	VAL	ASN	HIS	ARG	ALA	GLU	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	LYS
8	ILE	ALA	ALA	ALA	ASN	VAL	PRO	ALA	PHE	VAL
9	SER	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	LYS

1	MET	ASN	LYS	SER	GLN	LEU	ILE	ASP	LYS	ILE
2	ALA	ALA	GLY	ALA	ASP	ILE	SER	LYS	ALA	ALA
3	ALA	GLY	ARG	ALA	LEU	ASP	ALA	ILE	ILE	ALA
4	SER	VAL	THR	GLU	SER	LEU	LYS	GLU	GLY	ASP
5	ASP	VAL	ALA	LEU	VAL	GLY	PHE	GLY	THR	PHE
6	ALA	VAL	LYS	GLU	ARG	ALA	ALA	ARG	THR	GLY
7	ARG	ASN	PRO	GLN	THR	GLY	LYS	GLU	ILE	THR
8	ILE	ALA	ALA	ALA	LYS	VAL	PRO	SER	PHE	ARG
9	ALA	GLY	LYS	ALA	LEU	LYS	ASP	ALA	VAL	ASN