BMRB Entry 25301

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25301

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Title:
Ig1 domain of human obscurin
Deposition date:
2014-10-28
Original release date:
2015-08-25
Authors:
Wright, Nathan; Rudloff, Michael; Woosley, Alec
Citation:

Citation: Rudloff, Michael; Woosley, Alec; Wright, Nathan. "Biophysical characterization of naturally occurring titin M10 mutations"  Protein Sci. 24, 946-955 (2015).
PubMed: 25739468

Assembly members:

Assembly members:
Ig1, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ig1: MDQPQFSGAPRFLTRPKAFV VSVGKDATLSCQIVGNPTPQ VSWEKDQQPVTAGARFRLAQ DGDLYRLTILDLALGDSGQY VCRARNAIGEAFAAVGLQVD AEAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts263
15N chemical shifts78
1H chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ig11

Entities:

Entity 1, Ig1 112 residues - Formula weight is not available

1   METASPGLNPROGLNPHESERGLYALAPRO
2   ARGPHELEUTHRARGPROLYSALAPHEVAL
3   VALSERVALGLYLYSASPALATHRLEUSER
4   CYSGLNILEVALGLYASNPROTHRPROGLN
5   VALSERTRPGLULYSASPGLNGLNPROVAL
6   THRALAGLYALAARGPHEARGLEUALAGLN
7   ASPGLYASPLEUTYRARGLEUTHRILELEU
8   ASPLEUALALEUGLYASPSERGLYGLNTYR
9   VALCYSARGALAARGASNALAILEGLYGLU
10   ALAPHEALAALAVALGLYLEUGLNVALASP
11   ALAGLUALAALALEUGLUHISHISHISHIS
12   HISHIS

Related Database Links:

BMRB 25304
PDB 4C4K 4UOW

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