BMRB Entry 25278

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25278

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Title:
Backbone chemical shift assignments for the sensor domain of the Burkholderia pseudomallei histidine kinase RisS. Seattle Structural Genomics Center for Infectious Disease target BupsA.00863.i.
Deposition date:
2014-10-09
Original release date:
2014-11-11
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Edwards, Thomas; Hewitt, Stephen; Phan, Isabelle; Van Voorhis, Wesley; Miller, Samuel; Myler, Peter. "Backbone chemical shift assignments for the sensor domain of the Burkholderia pseudomallei histidine kinase RisS - An "invisible" dimer interface."  .

Assembly members:

Assembly members:
RisS, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: b-proteobacteria   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia pseudomallei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RisS: GPGSMSFRVIEREPRAQRVA LQLVAIVKLTRTALLYSDPD LRRALLQDLESNEGVRVYPR EKTDKFKLQPDESVNRLIEH DIRSRLGDDTVIAQSVNDIP GVWISFKIDDDDYWVALDRD QLDTVTG

Data sets:
Data typeCount
13C chemical shifts247
1H chemical shifts75
15N chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 127 residues - Formula weight is not available

1   GLYPROGLYSERMETSERPHEARGVALILE
2   GLUARGGLUPROARGALAGLNARGVALALA
3   LEUGLNLEUVALALAILEVALLYSLEUTHR
4   ARGTHRALALEULEUTYRSERASPPROASP
5   LEUARGARGALALEULEUGLNASPLEUGLU
6   SERASNGLUGLYVALARGVALTYRPROARG
7   GLULYSTHRASPLYSPHELYSLEUGLNPRO
8   ASPGLUSERVALASNARGLEUILEGLUHIS
9   ASPILEARGSERARGLEUGLYASPASPTHR
10   VALILEALAGLNSERVALASNASPILEPRO
11   GLYVALTRPILESERPHELYSILEASPASP
12   ASPASPTYRTRPVALALALEUASPARGASP
13   GLNLEUASPTHRVALTHRGLY

Related Database Links:

PDB 3LR0 3LR3 3LR4 3LR5 3TE8
DBJ BAG43831
EMBL CAH36098 CAR52312 CDN60458 CDU28740 CFB52363
GB AAU47661 ABA50560 ABB08844 ABC38194 ABF81004
REF WP_004196632 WP_004531418 WP_006402221 WP_006408378 WP_006415644

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks