BMRB Entry 25277

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PDB ID: 2mvo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25277
MolProbity Validation Chart

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Title:
Solution structure of the lantibiotic self-resistance lipoprotein MlbQ from Microbispora ATCC PTA-5024
Deposition date:
2014-10-09
Original release date:
2015-07-13
Authors:
Pozzi, Roberta; Schwartz, Paul; Linke, Dirk; Kulik, Andreas; Nega, Mulugeta; Wohlleben, Wolfgang; Stegmann, Evi; Coles, Murray
Citation:

Citation: Pozzi, Roberta; Coles, Murray; Schwartz, Paul; Linke, Dirk; Kulik, Andreas; Nega, Mulugeta; Wohlleben, Wolfgang; Stegmann, Evi. "Distinct mechanisms contribute to immunity in the lantibiotic NAI-107 producer strain Microbispora ATCC PTA-5024"  Environ. Microbiol. 18, 118-132 (2016).
PubMed: 25923468

Assembly members:

Assembly members:
MlbQ, polymer, 147 residues, 15456.215 Da.

Natural source:

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 316330   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Microbispora ATCC PTA-5024

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MlbQ: HHHHHHSSGLVPRGSGMKET AAAKFERQHMDSPDLGTDDD DKTGGGRADPAHRSPVPLPS PTSNKQDISEANLAYLWPLT VDHGTIECLPSDNAVFVAPD GTTYALNDRAEKAGHPPITP IRAKGSGGGYISLGALLSTT LNLCGKG

Data sets:
Data typeCount
13C chemical shifts347
15N chemical shifts82
1H chemical shifts573

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1MlbQ1

Entities:

Entity 1, MlbQ 147 residues - 15456.215 Da.

Residues -5-36 represent a non-native cloning/affinity tag

1   HISHISHISHISHISHISSERSERGLYLEU
2   VALPROARGGLYSERGLYMETLYSGLUTHR
3   ALAALAALALYSPHEGLUARGGLNHISMET
4   ASPSERPROASPLEUGLYTHRASPASPASP
5   ASPLYSTHRGLYGLYGLYARGALAASPPRO
6   ALAHISARGSERPROVALPROLEUPROSER
7   PROTHRSERASNLYSGLNASPILESERGLU
8   ALAASNLEUALATYRLEUTRPPROLEUTHR
9   VALASPHISGLYTHRILEGLUCYSLEUPRO
10   SERASPASNALAVALPHEVALALAPROASP
11   GLYTHRTHRTYRALALEUASNASPARGALA
12   GLULYSALAGLYHISPROPROILETHRPRO
13   ILEARGALALYSGLYSERGLYGLYGLYTYR
14   ILESERLEUGLYALALEULEUSERTHRTHR
15   LEUASNLEUCYSGLYLYSGLY

Related Database Links:

GB ETK34049.1

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