BMRB Entry 25260

Name: Solution structure of human insulin at pH 1.9
Published: 2014-12-08

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PDB ID: 2mvc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25260
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of human insulin at pH 1.9

Deposition date:

2014-10-02

Original release date:

2014-12-08

Authors:

Hexnerova, Rozalie; Krizkova, Kvetoslava; Maletinska, Lenka; Jiracek, Jiri; Brzozowski, Andrzej; Zakova, Lenka; Veverka, Vaclav

Citation:

Citation: Krizkova, Kvetoslava; Veverka, Vaclav; Maletinska, Lenka; Hexnerova, Rozalie; Brzozowski, Andrzej; Jiracek, Jiri; Zakova, Lenka. "Structural and Functional Study of the GlnB22-Insulin Mutant Responsible for Maturity-Onset Diabetes of the Young" Plos ONE 9, e112883-e112883 (2014).
PubMed: 25423173

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, 2383.700 Da.
entity_2, polymer, 30 residues, 3433.981 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GIVEQCCTSICSLYQLENYC N
entity_2: FVNQHLCGSHLVEALYLVCG ERGFFYTPKT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	131
15N chemical shifts	40
1H chemical shifts	355

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 21 residues - 2383.700 Da.

1

GLY

ILE

VAL

GLU

GLN

CYS

THR

SER

ILE

2

CYS

SER

LEU

TYR

GLN

LEU

GLU

ASN

TYR

CYS

3

ASN

Entity 2, entity_2 30 residues - 3433.981 Da.

1	PHE	VAL	ASN	GLN	HIS	LEU	CYS	GLY	SER	HIS
2	LEU	VAL	GLU	ALA	LEU	TYR	LEU	VAL	CYS	GLY
3	GLU	ARG	GLY	PHE	PHE	TYR	THR	PRO	LYS	THR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 25260

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	PHE	VAL	ASN	GLN	HIS	LEU	CYS	GLY	SER	HIS
2	LEU	VAL	GLU	ALA	LEU	TYR	LEU	VAL	CYS	GLY
3	GLU	ARG	GLY	PHE	PHE	TYR	THR	PRO	LYS	THR

1	PHE	VAL	ASN	GLN	HIS	LEU	CYS	GLY	SER	HIS
2	LEU	VAL	GLU	ALA	LEU	TYR	LEU	VAL	CYS	GLY
3	GLU	ARG	GLY	PHE	PHE	TYR	THR	PRO	LYS	THR

1	PHE	VAL	ASN	GLN	HIS	LEU	CYS	GLY	SER	HIS
2	LEU	VAL	GLU	ALA	LEU	TYR	LEU	VAL	CYS	GLY
3	GLU	ARG	GLY	PHE	PHE	TYR	THR	PRO	LYS	THR