BMRB Entry 25250

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PDB ID: 2mv8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25250
MolProbity Validation Chart

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Title:
Solution structure of Ovis Aries PrP with mutation delta190-197
Deposition date:
2014-09-25
Original release date:
2015-10-05
Authors:
Munoz, Carola; Egalon, Angelique; Beringue, Vincent; Rezaei, Human; Dron, Michel; Sizun, Christina
Citation:

Citation: Munoz, Carola; Sizun, Christina; Egalon, Angelique; Beringue, Vincent; Rezaei, Human; Dron, Michel. "Conversion ability of PrP helix 2 deletion mutants"  .

Assembly members:

Assembly members:
PrPdelta190-197, polymer, 146 residues, 16655.6213 Da.

Natural source:

Natural source:   Common Name: Sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PrPdelta190-197: MGSSHHHHHHSSGLVPRGSH MSNKPSKPKTNMKHVAGAAA AGAVVGGLGGYMLGSVMSRP LIHFGNDYEDRYYRENMYRY PNQVYYRPVDQYSNQNNFVH DCVNITVKQGENFTETDIKI MERVVEQMCITQYQRESQAY YQRGAS

Data sets:
Data typeCount
13C chemical shifts569
15N chemical shifts141
1H chemical shifts898

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 146 residues - 16655.6213 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASNLYSPROSERLYSPROLYSTHR
4   ASNMETLYSHISVALALAGLYALAALAALA
5   ALAGLYALAVALVALGLYGLYLEUGLYGLY
6   TYRMETLEUGLYSERVALMETSERARGPRO
7   LEUILEHISPHEGLYASNASPTYRGLUASP
8   ARGTYRTYRARGGLUASNMETTYRARGTYR
9   PROASNGLNVALTYRTYRARGPROVALASP
10   GLNTYRSERASNGLNASNASNPHEVALHIS
11   ASPCYSVALASNILETHRVALLYSGLNGLY
12   GLUASNPHETHRGLUTHRASPILELYSILE
13   METGLUARGVALVALGLUGLNMETCYSILE
14   THRGLNTYRGLNARGGLUSERGLNALATYR
15   TYRGLNARGGLYALASER

Related Database Links:

GenBank CAA04276
GB AJ000738.1

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