BMRB Entry 25241

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PDB ID: 2mv3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25241
MolProbity Validation Chart

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Title:
The N-domain of the AAA metalloproteinase Yme1 from Saccharomyces cerevisiae
Deposition date:
2014-09-22
Original release date:
2015-01-26
Authors:
Scharfenberg, Franka; Serek-Heuberger, Justyna; Martin, Joerg; Lupas, Andrei; Coles, Murray
Citation:

Citation: Scharfenberg, Franka; Serek-Heuberger, Justyna; Coles, Murray; Hartmann, Marcus; Habeck, Michael; Martin, Joerg; Lupas, Andrei; Alva, Vikram. "Structure and Evolution of N-domains in AAA Metalloproteases"  J. Mol. Biol. 427, 910-923 (2015).
PubMed: 25576874

Assembly members:

Assembly members:
Yme1-N, polymer, 88 residues, 10056.332 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yme1-N: MVAVSHAMLATREQEANKDL TSPDAQAAFYKLLLQSNYPQ YVVSRFETPGIASSPECMEL YMEALQRIGRHSEADAVRQN LEHHHHHH

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts56
1H chemical shifts512

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Yme1-N1

Entities:

Entity 1, Yme1-N 88 residues - 10056.332 Da.

Residues E177-H183 are a non-native affinity tag, residue M96 is a cloning artefact

1   METVALALAVALSERHISALAMETLEUALA
2   THRARGGLUGLNGLUALAASNLYSASPLEU
3   THRSERPROASPALAGLNALAALAPHETYR
4   LYSLEULEULEUGLNSERASNTYRPROGLN
5   TYRVALVALSERARGPHEGLUTHRPROGLY
6   ILEALASERSERPROGLUCYSMETGLULEU
7   TYRMETGLUALALEUGLNARGILEGLYARG
8   HISSERGLUALAASPALAVALARGGLNASN
9   LEUGLUHISHISHISHISHISHIS

Related Database Links:

NCBI NP_015349.1

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