BMRB Entry 25232

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PDB ID: 2mut
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25232
MolProbity Validation Chart

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Title:
Solution structure of the F231L mutant ERCC1-XPF dimerization region
Deposition date:
2014-09-17
Original release date:
2015-06-22
Authors:
Faridounnia, Maryam; Wienk, Hans; Kovacic, Lidija; Folkers, Gert; Jaspers, Nicolaas; Kaptein, Robert; Hoeijmakers, Jan; Boelens, Rolf
Citation:

Citation: Faridounnia, Maryam; Wienk, Hans; Kovacic, Lidija; Folkers, Gert; Jaspers, Nicolaas; Boelens, Rolf. "The Cerebro-Oculo-Facio-Skeletal (COFS) Syndrome point mutation F231L in the ERCC1 DNA repair protein causes dissociation of the ERCC1-XPF complex"  J. Biol. Chem. ., .-. (2015).
PubMed: 26085086

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, 10985.764 Da.
entity_2, polymer, 84 residues, 9230.592 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RIRRRYNMADLLMEKLEQDL VSRVTECLTTVKSVNKTDSQ TLLTTFGSLEQLIAASREDL ALCPGLGPQKARRLFDVLHE PFLKVPGGLEHHHHHH
entity_2: MDSETLPESEKYNPGPQDFL LKMPGVNAKNCRSLMHHVKN IAELAALSQDELTSILGNAA NAKQLYDFIHTSFAEVVSKG KGKK

Data sets:
Data typeCount
13C chemical shifts695
15N chemical shifts172
1H chemical shifts1173

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 96 residues - 10985.764 Da.

1   ARGILEARGARGARGTYRASNMETALAASP
2   LEULEUMETGLULYSLEUGLUGLNASPLEU
3   VALSERARGVALTHRGLUCYSLEUTHRTHR
4   VALLYSSERVALASNLYSTHRASPSERGLN
5   THRLEULEUTHRTHRPHEGLYSERLEUGLU
6   GLNLEUILEALAALASERARGGLUASPLEU
7   ALALEUCYSPROGLYLEUGLYPROGLNLYS
8   ALAARGARGLEUPHEASPVALLEUHISGLU
9   PROPHELEULYSVALPROGLYGLYLEUGLU
10   HISHISHISHISHISHIS

Entity 2, entity_2 84 residues - 9230.592 Da.

1   METASPSERGLUTHRLEUPROGLUSERGLU
2   LYSTYRASNPROGLYPROGLNASPPHELEU
3   LEULYSMETPROGLYVALASNALALYSASN
4   CYSARGSERLEUMETHISHISVALLYSASN
5   ILEALAGLULEUALAALALEUSERGLNASP
6   GLULEUTHRSERILELEUGLYASNALAALA
7   ASNALALYSGLNLEUTYRASPPHEILEHIS
8   THRSERPHEALAGLUVALVALSERLYSGLY
9   LYSGLYLYSLYS

Related Database Links:

PDB 1Z00 2A1J 2MUT
DBJ BAG37398 BAG52472
GB AAA35810 AAA52394 AAC16253 AAH08930 AAM34796
PRF 1403276A
REF NP_001181860 NP_001974 XP_003817569 XP_003915760 XP_003915761
SP P07992
AlphaFold P07992 Q8TD83

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SPARKY: Backbone or all simulated peaks