BMRB Entry 25195

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PDB ID: 2mu0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25195
MolProbity Validation Chart

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Title:
Solution structure of a putative arsenate reductase from Brucella melitensis. Seattle Structural Genomics Center for Infectious Disease target BrabA.00073.a
Deposition date:
2014-09-02
Original release date:
2014-09-15
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Hewitt, Stephan; Van Voorhis, Wesley; Myler, Peter. "Solution NMR structure of an arsenate reductase from Brucella melitensis."  .

Assembly members:

Assembly members:
entity, polymer, 136 residues, 15166.851 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 29459   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Brucella melitensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAHHHHHHMGTLEAQTQGPG SMDVTIYHNPVCGTSRKVLG MIREAGIEPHVIEYMKTPLP RDMLVELLRQMAISPRALLR AKEARYAELGLDDPALSDEV LIDAMISNPVLMNRPVVVTP KGVRLCRPAETVQELL

Data sets:
Data typeCount
13C chemical shifts524
1H chemical shifts801
15N chemical shifts115

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 136 residues - 15166.851 Da.

The 21 residues at the N-terminus are not natural.

1   METALAHISHISHISHISHISHISMETGLY
2   THRLEUGLUALAGLNTHRGLNGLYPROGLY
3   SERMETASPVALTHRILETYRHISASNPRO
4   VALCYSGLYTHRSERARGLYSVALLEUGLY
5   METILEARGGLUALAGLYILEGLUPROHIS
6   VALILEGLUTYRMETLYSTHRPROLEUPRO
7   ARGASPMETLEUVALGLULEULEUARGGLN
8   METALAILESERPROARGALALEULEUARG
9   ALALYSGLUALAARGTYRALAGLULEUGLY
10   LEUASPASPPROALALEUSERASPGLUVAL
11   LEUILEASPALAMETILESERASNPROVAL
12   LEUMETASNARGPROVALVALVALTHRPRO
13   LYSGLYVALARGLEUCYSARGPROALAGLU
14   THRVALGLNGLULEULEU

Related Database Links:

PDB 2MU0
EMBL CAJ10964 CDL76386
GB AAL52173 AAN29912 AAX74345 ABQ61470 ABX62059
REF WP_002964107 WP_004683746 WP_004685628 WP_004691862 WP_006012423

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