BMRB Entry 25178
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25178
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Citation: Cook, Gabriel; Dawson, Lindsay; Tian, Ye; Opella, Stanley. "Three-dimensional structure of the p7 protein of hepatitis C virus in phospholipid bilayers by solid-state NMR" Biochemistry ., .-..
PubMed: 23841474
Assembly members:
entity, polymer, 63 residues, 6693.997 Da.
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 420174 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHLV
Entity Sequences (FASTA):
entity: ALENLVVLNAASVAGAHGIL
SFLVFFSAAWYIKGRLAPGA
AYAFYGVWPLLLLLLALPPR
AYA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 86 |
| 15N chemical shifts | 47 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 63 residues - 6693.997 Da.
| 1 | ALA | LEU | GLU | ASN | LEU | VAL | VAL | LEU | ASN | ALA | ||||
| 2 | ALA | SER | VAL | ALA | GLY | ALA | HIS | GLY | ILE | LEU | ||||
| 3 | SER | PHE | LEU | VAL | PHE | PHE | SER | ALA | ALA | TRP | ||||
| 4 | TYR | ILE | LYS | GLY | ARG | LEU | ALA | PRO | GLY | ALA | ||||
| 5 | ALA | TYR | ALA | PHE | TYR | GLY | VAL | TRP | PRO | LEU | ||||
| 6 | LEU | LEU | LEU | LEU | LEU | ALA | LEU | PRO | PRO | ARG | ||||
| 7 | ALA | TYR | ALA |
Samples:
sample_1: p7, [U-100% 13C; U-100% 15N], 3 – 4 mg; HEPES 20 mM; H2O 100%; DMPC 25 mg
sample_conditions_1: pH: 7.3; temperature: 298 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N-13C HETCOR | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C/13C Correlation | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Xplor-NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution
NMR spectrometers:
- Varian INOVA 700 MHz