BMRB Entry 25175

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PDB ID: 2n32
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25175
MolProbity Validation Chart

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Title:
NMR resonance assignment of the N-terminal domain of the lantibiotic immunity protein NisI
Deposition date:
2014-08-26
Original release date:
2015-04-27
Authors:
Hacker, Carolin; Christ, Nina Alexandra; Duchardt-Ferner, Elke; Bernigner, Lucija; Koetter, Peter; Entian, Karl-Dieter; Woehnert, Jens
Citation:

Citation: Hacker, Carolin; Christ, Nina Alexandra; Duchardt-Ferner, Elke; Bernigner, Lucija; Koetter, Peter; Entian, Karl-Dieter; Woehnert, Jens. "NMR resonance assignments of the lantibiotic immunity protein NisI from Lactococcus lactis"  Biomol NMR Assign. 9, 293-297 (2015).
PubMed: 25613223

Assembly members:

Assembly members:
NisI, polymer, 109 residues, 12779.5 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NisI: YQTSHKKVRFDEGSYTNFIY DNKSYFVTDKEIPQENVNNS KVKFYKLLIVDMKSEKLLSS SNKNSVTLVLNNIYEASDKS LCMGINDRYYKILPESDKGA VKALRLQNF

Data sets:
Data typeCount
13C chemical shifts493
15N chemical shifts117
1H chemical shifts783

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1NisI1

Entities:

Entity 1, NisI 109 residues - 12779.5 Da.

1   TYRGLNTHRSERHISLYSLYSVALARGPHE
2   ASPGLUGLYSERTYRTHRASNPHEILETYR
3   ASPASNLYSSERTYRPHEVALTHRASPLYS
4   GLUILEPROGLNGLUASNVALASNASNSER
5   LYSVALLYSPHETYRLYSLEULEUILEVAL
6   ASPMETLYSSERGLULYSLEULEUSERSER
7   SERASNLYSASNSERVALTHRLEUVALLEU
8   ASNASNILETYRGLUALASERASPLYSSER
9   LEUCYSMETGLYILEASNASPARGTYRTYR
10   LYSILELEUPROGLUSERASPLYSGLYALA
11   VALLYSALALEUARGLEUGLNASNPHE

Related Database Links:

BMRB 25193
PDB 2N32
DBJ BAL50562
EMBL CAA54209
GB AAA25193 AAQ89591 AAQ89592 ADJ56356 ADZ63253
REF WP_014570409 WP_015425983 WP_039114828 WP_042748180
SP P42708
AlphaFold P42708

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