BMRB Entry 25172

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25172

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Title:
H, 13C and 15N assignments of EGF domains 4 to 7 of human Notch-1
Deposition date:
2014-08-25
Original release date:
2016-06-30
Authors:
Handford, Penny; Redfield, Christina; Weisshuhn, Philip
Citation:

Citation: Weisshuhn, Philip; Sheppard, Devon; Taylor, Paul; Whiteman, Pat; Lea, Susan; Handford, Penny; Redfield, Christina. "Non-Linear and Flexible Regions of the Human Notch1 Extracellular Domain Revealed by High-Resolution Structural Studies"  Structure 24, 555-566 (2016).
PubMed: 26996961

Assembly members:

Assembly members:
hN-1_4-7, polymer, 157 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hN-1_4-7: SAQADPCASNPCANGGQCLP FEASYICHCPPSFHGPTCRQ DVNECGQKPGLCRHGGTCHN EVGSYRCVCRATHTGPNCER PYVPCSPSPCQNGGTCRPTG DVTHECACLPGFTGQNCEEN IDDCPGNNCKNGGACVDGVN TYNCRCPPEWTGQYCTE

Data sets:
Data typeCount
13C chemical shifts489
15N chemical shifts146
1H chemical shifts752

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hN-1 4-71
2Ca2+, 12
3Ca2+, 22

Entities:

Entity 1, hN-1 4-7 157 residues - Formula weight is not available

1   SERALAGLNALAASPPROCYSALASERASN
2   PROCYSALAASNGLYGLYGLNCYSLEUPRO
3   PHEGLUALASERTYRILECYSHISCYSPRO
4   PROSERPHEHISGLYPROTHRCYSARGGLN
5   ASPVALASNGLUCYSGLYGLNLYSPROGLY
6   LEUCYSARGHISGLYGLYTHRCYSHISASN
7   GLUVALGLYSERTYRARGCYSVALCYSARG
8   ALATHRHISTHRGLYPROASNCYSGLUARG
9   PROTYRVALPROCYSSERPROSERPROCYS
10   GLNASNGLYGLYTHRCYSARGPROTHRGLY
11   ASPVALTHRHISGLUCYSALACYSLEUPRO
12   GLYPHETHRGLYGLNASNCYSGLUGLUASN
13   ILEASPASPCYSPROGLYASNASNCYSLYS
14   ASNGLYGLYALACYSVALASPGLYVALASN
15   THRTYRASNCYSARGCYSPROPROGLUTRP
16   THRGLYGLNTYRCYSTHRGLU

Entity 2, Ca2+, 1 - Ca - 40.078 Da.

1   CA

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