BMRB Entry 25135

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PDB ID: 2msv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25135
MolProbity Validation Chart

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Title:
Solution structure of the MLKL N-terminal domain
Deposition date:
2014-08-07
Original release date:
2014-09-22
Authors:
Su, Lijing; Rizo, Josep; Quade, Bradley; Wang, Huayi; Sun, Liming; Wang, Xiaodong
Citation:

Citation: Su, Lijing; Quade, Bradley; Wang, Huayi; Sun, Liming; Wang, Xiaodong; Rizo, Josep. "A Plug Release Mechanism for Membrane Permeation by MLKL"  Structure ., .-..

Assembly members:

Assembly members:
entity, polymer, 166 residues, 17999.953 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSPGISGGGGGILENLKHII TLGQVIHKRCEEMKYCKKQC RRLGHRVLGLIKPLEMLQDQ GKRSVPSEKLTTAMNRFKAA LEEANGEIEKFSNRSNICRF LTASQDKILFKDVNRKLSDV WKELSLLLQVEQRMPVSPIS QGASWAQEDQQDADEDRRAF QMLRRD

Data sets:
Data typeCount
15N chemical shifts169
1H chemical shifts1135
13C chemical shifts690

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 166 residues - 17999.953 Da.

Residues 1-12 arise from a linker to a non-native affinity tag. Residue 13 (Leu) also comes from the tag and replaces the native Met1

1   GLYSERPROGLYILESERGLYGLYGLYGLY
2   GLYILELEUGLUASNLEULYSHISILEILE
3   THRLEUGLYGLNVALILEHISLYSARGCYS
4   GLUGLUMETLYSTYRCYSLYSLYSGLNCYS
5   ARGARGLEUGLYHISARGVALLEUGLYLEU
6   ILELYSPROLEUGLUMETLEUGLNASPGLN
7   GLYLYSARGSERVALPROSERGLULYSLEU
8   THRTHRALAMETASNARGPHELYSALAALA
9   LEUGLUGLUALAASNGLYGLUILEGLULYS
10   PHESERASNARGSERASNILECYSARGPHE
11   LEUTHRALASERGLNASPLYSILELEUPHE
12   LYSASPVALASNARGLYSLEUSERASPVAL
13   TRPLYSGLULEUSERLEULEULEUGLNVAL
14   GLUGLNARGMETPROVALSERPROILESER
15   GLNGLYALASERTRPALAGLNGLUASPGLN
16   GLNASPALAASPGLUASPARGARGALAPHE
17   GLNMETLEUARGARGASP

Related Database Links:

NCBI NP_689862.1
PDB 2MSV
DBJ BAC03728 BAI46196
EMBL CAI46144
GB AAH28141 AAX41010 AIC53362 EAW95679
REF NP_001135969 NP_689862 XP_005255891 XP_008953137 XP_008953138
SP Q8NB16
AlphaFold Q8NB16

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks