BMRB Entry 25066

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PDB ID: 2mr9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25066
MolProbity Validation Chart

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Title:
NMR structure of UBA domain of DNA-damage-inducible 1 protein (Ddi1)
Deposition date:
2014-07-02
Original release date:
2015-02-02
Authors:
Zhang, Daoning; Fushman, David
Citation:

Citation: Nowicka, Urszula; Zhang, Daoning; Walker, Olivier; Castaneda, Calos; Krutauz, Daria; Chen, Tony; Reis, Noa; Glickman, Michael; Fushman, David. "DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain"  Structure ., .-..

Assembly members:

Assembly members:
entity, polymer, 44 residues, 4745.415 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSATFPEQTIKQLMDLGFPR DAVVKALKQTNGNAEFAASL LFQS

Data sets:
Data typeCount
1H chemical shifts296
15N chemical shifts45

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1UBA domain of DNA-damage-inducible 1 protein (Ddi1)1

Entities:

Entity 1, UBA domain of DNA-damage-inducible 1 protein (Ddi1) 44 residues - 4745.415 Da.

First three residues 'GSA' represent a non-native affinity tag. Residue 4T represents 389T in the Ddi1 sequence.

1   GLYSERALATHRPHEPROGLUGLNTHRILE
2   LYSGLNLEUMETASPLEUGLYPHEPROARG
3   ASPALAVALVALLYSALALEULYSGLNTHR
4   ASNGLYASNALAGLUPHEALAALASERLEU
5   LEUPHEGLNSER

Related Database Links:

GB EDN63120.1 AAB64670 AAB82066 AAC18522 AAT92964 AHY75700
PDB 2MR9 2MRO
DBJ GAA22973
EMBL CAY79329
REF NP_011070
SP P40087
TPG DAA07804
AlphaFold P40087

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks