BMRB Entry 25037

Name: Solution structure of N terminal domain of the MuB AAA+ ATPase
Published: 2015-06-29

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PDB ID: 2mqk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25037
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of N terminal domain of the MuB AAA+ ATPase

Deposition date:

2014-06-23

Original release date:

2015-06-29

Authors:

Lopez-Mendez, Blanca; Dramicanin, Marija; Campos-Olivas, Ramon; Ramon-Maiques, Santiago

Citation:

Citation: Dramicanin, Marija; Lopez-Mendez, Blanca; Campos-Olivas, Ramon; Ramon-Maiques, Santiago. "Solution structure of N terminal domain of the MuB AAA+ ATPase" To be Published ., .-..

Assembly members:

Assembly members:
MuB-NTD, polymer, 65 residues, 7353.184 Da.

Natural source:

Natural source: Common Name: Enterobacteria phage Mu Taxonomy ID: 10677 Superkingdom: Viruses Kingdom: not available Genus/species: bacteriophage Mu

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pOPINB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MuB-NTD: GPMNISDIRAGLRTLVENEE TTFKQIALESGLSTGTISSF INDKYNGDNERVSQTLQRWL EKYHA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	183
15N chemical shifts	68
1H chemical shifts	454

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MuB-NTD	1

Entities:

Entity 1, MuB-NTD 65 residues - 7353.184 Da.

1

GLY

PRO

MET

ASN

ILE

SER

ASP

ILE

ARG

ALA

2

GLY

LEU

ARG

THR

LEU

VAL

GLU

ASN

GLU

3

THR

PHE

LYS

GLN

ILE

ALA

LEU

GLU

SER

4

GLY

LEU

SER

THR

GLY

THR

ILE

SER

PHE

5

ILE

ASN

ASP

LYS

TYR

ASN

GLY

ASP

ASN

GLU

6

ARG

VAL

SER

GLN

THR

LEU

GLN

ARG

TRP

LEU

7

GLU

LYS

TYR

HIS

ALA

Samples:

sample_1: MuB-NTD 500 uM; sodium phosphate 20 mM; sodium chloride 100 mM

sample_2: MuB-NTD, [U-98% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.2.6, Guntert, Mumenthaler and Wuthrich - structure solution

OPAL v1.4, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN v2.3.1, CCPN - chemical shift assignment, data analysis, peak picking

TOPSPIN v2.1, Bruker Biospin - collection, processing

TALOS+, Cornilescu, Delaglio and Bax - backbone torsion angle restraint generation

NMR spectrometers:

Bruker Avance 700 MHz

PDB	2MQK
EMBL	CAA25599 CDP68213 CDU35404 CTU52180 CTV64249
GB	AAA32370 AAA32382 AAF01100 AAW58941 ACV50263
REF	NP_050608 WP_001026707 WP_001026708 WP_001026709 WP_001026710
SP	P03763 P13771
AlphaFold	P03763 P13771