BMRB Entry 25000

Name: WW3 domain of Nedd4L in complex with its HECT domain PY motif
Published: 2014-10-20

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PDB ID: 2mpt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25000
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

WW3 domain of Nedd4L in complex with its HECT domain PY motif

Deposition date:

2014-06-02

Original release date:

2014-10-20

Authors:

Escobedo, Albert; Macias, Maria; Gomes, Tiago; Aragon, Eric; Martin-Malpartida, Pau; Ruiz, Lidia

Citation:

Citation: Escobedo, Albert; Gomes, Tiago; Aragon, Eric; Martin-Malpartida, Pau; Ruiz, Lidia; Macias, Maria. "Structural Basis of the Activation and Degradation Mechanisms of the E3 Ubiquitin Ligase Nedd4L" Structure 22, 1446-1457 (2014).
PubMed: 25295397

Assembly members:

Assembly members:
WW3, polymer, 48 residues, 3833.352 Da.
HECT_PY, polymer, 16 residues, 1608.784 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WW3: GAMEQSFLPPGWEMRIAPNG RPFFIDHNTKTTTWEDPRLK FPVHMRSK
HECT_PY: RLDLPPYETFEDLREK

Data sets:

assigned_chemical_shifts

Data type	Count
1H chemical shifts	280
13C chemical shifts	59
15N chemical shifts	27

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WW3	1
2	HECT_PY	2

Entities:

Entity 1, WW3 48 residues - 3833.352 Da.

Sequence numbering as in isoform 5

1

GLY

ALA

MET

GLU

GLN

SER

PHE

LEU

PRO

2

GLY

TRP

GLU

MET

ARG

ILE

ALA

PRO

ASN

GLY

3

ARG

PRO

PHE

ILE

ASP

HIS

ASN

THR

LYS

4

THR

TRP

GLU

ASP

PRO

ARG

LEU

LYS

5

PHE

PRO

VAL

HIS

MET

ARG

SER

LYS

Entity 2, HECT_PY 16 residues - 1608.784 Da.

Sequence numbering as in isoform 5

1

ARG

LEU

ASP

LEU

PRO

TYR

GLU

THR

PHE

2

GLU

ASP

LEU

ARG

GLU

LYS

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1

UNP	Q96PU5 Q96PU5
BMRB	17529
PDB	1WR7 2LAJ 2MPT 2ONI 3JVZ 3JW0
DBJ	BAA23711 BAB69424 BAC31307 BAE37373 BAG11191
EMBL	CAB70754 CAH90908
GB	AAG43524 AAH00621 AAH19345 AAH32597 AAH39746
REF	NP_001008301 NP_001107858 NP_001125518 NP_001138436 NP_001138437
SP	Q5RBF2 Q8CFI0 Q96PU5
TPG	DAA15876
AlphaFold	Q9NT88 Q9NT88 Q5RBF2 Q8CFI0 Q96PU5

BMRB Entry 25000

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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