BMRB Entry 19859

Title:
Solution structure of the mature form, GK cecropin-like peptide from Ae. aegypti mosquito
Deposition date:
2014-03-16
Original release date:
2015-01-26
Authors:
Padilla, Andre; Misse, Dorothee
Citation:

Citation: Godreuil, Sylvain; Leban, Nadia; Padilla, Andre; Hamel, Rodolphe; Luplertlop, Natthanej; Chauffour, Aurelie; Vittecoq, Marion; Hoh, Francois; Thomas, Frederic; Sougakoff, Wladimir; Lionne, Corinne; Yssel, Hans; Misse, Dorothee. "Aedesin: structure and antimicrobial activity against multidrug resistant bacterial strains"  Plos One 9, e105441-e105441 (2014).
PubMed: 25162372

Assembly members:

Assembly members:
cecropin_GK, polymer, 36 residues, 3686.604 Da.

Natural source:

Natural source:   Common Name: yellow fever mosquito   Taxonomy ID: 7159   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aedes aegypti

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cecropin_GK: GGLKKLGKKLEGAGKRVFKA SEKALPVVVGIKAIGK

Data sets:
Data typeCount
13C chemical shifts53
15N chemical shifts27
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GK cecropin-like peptide1

Entities:

Entity 1, GK cecropin-like peptide 36 residues - 3686.604 Da.

Mature form of the immature, signal sequence-containing form MK

1   GLYGLYLEULYSLYSLEUGLYLYSLYSLEU
2   GLUGLYALAGLYLYSARGVALPHELYSALA
3   SERGLULYSALALEUPROVALVALVALGLY
4   ILELYSALAILEGLYLYS

Samples:

sample_1: cecropin GK 0.784 mM; Na phosphate 5.00 mM; K phosphate 0.88 mM; NaCl 68.50 mM; KCl 1.35 mM

sample_2: cecropin GK 0.784 mM; Na phosphate 5.00 mM; K phosphate 0.88 mM; NaCl 68.50 mM; KCl 1.35 mM

sample_conditions_1: ionic strength: 0.090 M; pH: 7.4; pressure: 1 atm; temperature: 283 K

sample_conditions_2: ionic strength: 0.090 M; pH: 7.4; pressure: 1 atm; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB EAT48342
REF XP_001649181

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks