BMRB Entry 19845

Name: Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins
Published: 2014-03-31

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PDB ID: 2mm4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19845
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins

Deposition date:

2014-03-09

Original release date:

2014-03-31

Authors:

Li, Yan; Surya, Wahyu; Claudine, Stephanie; Torres, Jaume

Citation:

Citation: Li, Yan; Surya, Wahyu; Claudine, Stephanie; Torres, Jaume. "Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins." J. Biol. Chem. 289, 12535-12549 (2014).
PubMed: 24668816

Assembly members:

Assembly members:
entity, polymer, 58 residues, 6066.473 Da.

Natural source:

Natural source: Common Name: Coronavirus Taxonomy ID: not available Superkingdom: viruses Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTBMalE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: ETGTLIVNSVLLFLAFVVFL LVTLAILTALRLAAYAANIV NVSLVKPTVYVYSRVKNL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	260
15N chemical shifts	63
1H chemical shifts	461

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Coronavirus Envelope Proteins-1	1

Entities:

Entity 1, Coronavirus Envelope Proteins-1 58 residues - 6066.473 Da.

1

GLU

THR

GLY

THR

LEU

ILE

VAL

ASN

SER

VAL

2

LEU

PHE

LEU

ALA

PHE

VAL

PHE

LEU

3

LEU

VAL

THR

LEU

ALA

ILE

LEU

THR

ALA

LEU

4

ARG

LEU

ALA

TYR

ALA

ASN

ILE

VAL

5

ASN

VAL

SER

LEU

VAL

LYS

PRO

THR

VAL

TYR

6

VAL

TYR

SER

ARG

VAL

LYS

ASN

LEU

Samples:

SARS_Etr: Coronavirus Envelope Proteins, [U-99% 13C; U-99% 15N], 0.8 mM; DSS 0.1 mM; D2O, [U-99% 2H], 5%; SDS 50 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	SARS_Etr	isotropic	sample_conditions_1
3D HNCA	SARS_Etr	isotropic	sample_conditions_1
3D HN(CO)CA	SARS_Etr	isotropic	sample_conditions_1
3D HNCO	SARS_Etr	isotropic	sample_conditions_1
3D HCCH-TOCSY	SARS_Etr	isotropic	sample_conditions_1
3D 1H-13C NOESY	SARS_Etr	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	SARS_Etr	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	SARS_Etr	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 700 MHz