BMRB Entry 19842

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19842

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Title:
Backbone and sidechain 1H, 13C, and 15N chemical shift assignments for the HEAT2 domain of human eIF4G
Deposition date:
2014-03-06
Original release date:
2016-11-28
Authors:
Edmonds, Katherine; Wagner, Gerhard
Citation:

Citation: Edmonds, Katherine; Wagner, Gerhard. "1H, 13C, and 15N backbone and sidechain chemical shift assignments for the HEAT2 domain of human eIF4GI"  Biomol. NMR Assignments 9, 157-160 (2015).
PubMed: 24929364

Assembly members:

Assembly members:
HEAT2_domain, polymer, 193 residues, 21600.1 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HEAT2_domain: GAALSEEELEKKSKAIIEEY LHLNDMKEAVQCVQELASPS LLFIFVRHGVESTLERSAIA REHMGQLLHQLLCAGHLSTA QYYQGLYEILELAEDMEIDI PHVWLYLAELVTPILQEGGV PMGELFREITKPLRPLGKAA SLLLEILGLLCKSMGPKKVG TLWREAGLSWKEFLPEGQDI GAFVAEQKVEYTL

Data sets:
Data typeCount
13C chemical shifts889
15N chemical shifts207
1H chemical shifts1451

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HEAT2 domain, 11
2HEAT2 domain, 21

Entities:

Entity 1, HEAT2 domain, 1 193 residues - 21600.1 Da.

1   GLYALAALALEUSERGLUGLUGLULEUGLU
2   LYSLYSSERLYSALAILEILEGLUGLUTYR
3   LEUHISLEUASNASPMETLYSGLUALAVAL
4   GLNCYSVALGLNGLULEUALASERPROSER
5   LEULEUPHEILEPHEVALARGHISGLYVAL
6   GLUSERTHRLEUGLUARGSERALAILEALA
7   ARGGLUHISMETGLYGLNLEULEUHISGLN
8   LEULEUCYSALAGLYHISLEUSERTHRALA
9   GLNTYRTYRGLNGLYLEUTYRGLUILELEU
10   GLULEUALAGLUASPMETGLUILEASPILE
11   PROHISVALTRPLEUTYRLEUALAGLULEU
12   VALTHRPROILELEUGLNGLUGLYGLYVAL
13   PROMETGLYGLULEUPHEARGGLUILETHR
14   LYSPROLEUARGPROLEUGLYLYSALAALA
15   SERLEULEULEUGLUILELEUGLYLEULEU
16   CYSLYSSERMETGLYPROLYSLYSVALGLY
17   THRLEUTRPARGGLUALAGLYLEUSERTRP
18   LYSGLUPHELEUPROGLUGLYGLNASPILE
19   GLYALAPHEVALALAGLUGLNLYSVALGLU
20   TYRTHRLEU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks