BMRB Entry 19782

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PDB ID: 6gcj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19782
MolProbity Validation Chart

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Title:
Chemical shifts assignments of the RodA hydrophobin from the opportunistic pathogen Aspergillus fumigatus
Deposition date:
2014-02-10
Original release date:
2019-03-18
Authors:
Pille, Ariane; Kwan, Ann H.; Cheung, Ivan; Hampsey, Matthew; Delepierre, Muriel; Sunde, Margaret; Guijarro, J. Inaki
Citation:

Citation: Pille, Ariane; Kwan, Ann H.; Cheung, Ivan; Hampsey, Matthew; Aimanianda, Vishukumar; Delepierre, Muriel; Latge, Jean-Paul; Sunde, Margaret; Guijarro, J. Inaki. "1H, 13C and 15N resonance assignments of the RodA hydrophobin from the opportunistic pathogen Aspergillus fumigatus"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
RodA, polymer, 142 residues, 14426.43 Da.

Natural source:

Natural source:   Common Name: ascomycetes   Taxonomy ID: 746128   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus fumigatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHUE-RodA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RodA: SLPQHDVNAAGNGVGNKGNA NVRFPVPDDITVKQATEKCG DQAQLSCCNKATYAGDVTDI DEGILAGTLKNLIGGGSGTE GLGLFNQCSKLDLQIPVIGI PIQALVNQKCKQNIACCQNS PSDASGSLIGLGLPCIALGS IL

Data sets:
Data typeCount
13C chemical shifts566
15N chemical shifts153
1H chemical shifts948

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RodA1

Entities:

Entity 1, RodA 142 residues - 14426.43 Da.

The recombinant protein consists of residues 19-159 of RodA and an additional N-terminal Ser residue. The 18 N-terminal residues coded by the RodA gene, which correspond to the secretion signal and are expected to be cleaved by A. fumigatus upon secretion, were not cloned.

1   SERLEUPROGLNHISASPVALASNALAALA
2   GLYASNGLYVALGLYASNLYSGLYASNALA
3   ASNVALARGPHEPROVALPROASPASPILE
4   THRVALLYSGLNALATHRGLULYSCYSGLY
5   ASPGLNALAGLNLEUSERCYSCYSASNLYS
6   ALATHRTYRALAGLYASPVALTHRASPILE
7   ASPGLUGLYILELEUALAGLYTHRLEULYS
8   ASNLEUILEGLYGLYGLYSERGLYTHRGLU
9   GLYLEUGLYLEUPHEASNGLNCYSSERLYS
10   LEUASPLEUGLNILEPROVALILEGLYILE
11   PROILEGLNALALEUVALASNGLNLYSCYS
12   LYSGLNASNILEALACYSCYSGLNASNSER
13   PROSERASPALASERGLYSERLEUILEGLY
14   LEUGLYLEUPROCYSILEALALEUGLYSER
15   ILELEU

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