BMRB Entry 19754

Name: Chemical shifts assignments of diacylglycerol kinase (DAGK) by solid-state NMR
Published: 2014-01-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19754

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts assignments of diacylglycerol kinase (DAGK) by solid-state NMR

Deposition date:

2014-01-27

Original release date:

2014-01-27

Authors:

Chen, Yanke; Zhang, Zhengfeng; Tang, Xinqi; Li, Jianping; Yang, Jun; Glaubitz, Clemens

Citation:

Citation: Chen, Yanke; Zhang, Zhengfeng; Tang, Xinqi; Li, Jianping; Glaubitz, Clemens; Yang, Jun. "Conformation and Topology of Diacylglycerol Kinase in E.coli Membranes Revealed by Solid-state NMR Spectroscopy." Angew. Chem. Int. Ed. Engl. ., .-. (2014).
PubMed: 24700682

Assembly members:

Assembly members:
DAGK, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSD005

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DAGK: MGHHHHHHELANNTTGFTRI IKAAGYSWKGLRAAWINEAA FRQEGVAVLLAVVIACWLDV DACTRVLLISSVMLVMIVEL LNSAIEAVVDRIGSEYHELS GRAKDMGSAAVLIAIIDAVI TWCILLWSHF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	369
15N chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DAGK	1

Entities:

Entity 1, DAGK 130 residues - Formula weight is not available

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	GLU	LEU
2	ALA	ASN	ASN	THR	THR	GLY	PHE	THR	ARG	ILE
3	ILE	LYS	ALA	ALA	GLY	TYR	SER	TRP	LYS	GLY
4	LEU	ARG	ALA	ALA	TRP	ILE	ASN	GLU	ALA	ALA
5	PHE	ARG	GLN	GLU	GLY	VAL	ALA	VAL	LEU	LEU
6	ALA	VAL	VAL	ILE	ALA	CYS	TRP	LEU	ASP	VAL
7	ASP	ALA	CYS	THR	ARG	VAL	LEU	LEU	ILE	SER
8	SER	VAL	MET	LEU	VAL	MET	ILE	VAL	GLU	LEU
9	LEU	ASN	SER	ALA	ILE	GLU	ALA	VAL	VAL	ASP
10	ARG	ILE	GLY	SER	GLU	TYR	HIS	GLU	LEU	SER
11	GLY	ARG	ALA	LYS	ASP	MET	GLY	SER	ALA	ALA
12	VAL	LEU	ILE	ALA	ILE	ILE	ASP	ALA	VAL	ILE
13	THR	TRP	CYS	ILE	LEU	LEU	TRP	SER	HIS	PHE

Name	Sample	Sample state	Sample conditions
3D NCACX	sample_1	solid	sample_conditions_1
3D NCOCX	sample_1	solid	sample_conditions_1
3D CONCA	sample_1	solid	sample_conditions_1
3D CAN(CO)CX	sample_1	solid	sample_conditions_1
3D CON(CA)CX	sample_1	solid	sample_conditions_1
3D CONCA	sample_2	solid	sample_conditions_1

PDB	2KDC 3ZE4 3ZE5 4CJZ 4CK0 4UP6 4UXW 4UXX
GB	EKW72221 ETJ14673

BMRB Entry 19754

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	GLU	LEU
2	ALA	ASN	ASN	THR	THR	GLY	PHE	THR	ARG	ILE
3	ILE	LYS	ALA	ALA	GLY	TYR	SER	TRP	LYS	GLY
4	LEU	ARG	ALA	ALA	TRP	ILE	ASN	GLU	ALA	ALA
5	PHE	ARG	GLN	GLU	GLY	VAL	ALA	VAL	LEU	LEU
6	ALA	VAL	VAL	ILE	ALA	CYS	TRP	LEU	ASP	VAL
7	ASP	ALA	CYS	THR	ARG	VAL	LEU	LEU	ILE	SER
8	SER	VAL	MET	LEU	VAL	MET	ILE	VAL	GLU	LEU
9	LEU	ASN	SER	ALA	ILE	GLU	ALA	VAL	VAL	ASP
10	ARG	ILE	GLY	SER	GLU	TYR	HIS	GLU	LEU	SER
11	GLY	ARG	ALA	LYS	ASP	MET	GLY	SER	ALA	ALA
12	VAL	LEU	ILE	ALA	ILE	ILE	ASP	ALA	VAL	ILE
13	THR	TRP	CYS	ILE	LEU	LEU	TRP	SER	HIS	PHE

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	GLU	LEU
2	ALA	ASN	ASN	THR	THR	GLY	PHE	THR	ARG	ILE
3	ILE	LYS	ALA	ALA	GLY	TYR	SER	TRP	LYS	GLY
4	LEU	ARG	ALA	ALA	TRP	ILE	ASN	GLU	ALA	ALA
5	PHE	ARG	GLN	GLU	GLY	VAL	ALA	VAL	LEU	LEU
6	ALA	VAL	VAL	ILE	ALA	CYS	TRP	LEU	ASP	VAL
7	ASP	ALA	CYS	THR	ARG	VAL	LEU	LEU	ILE	SER
8	SER	VAL	MET	LEU	VAL	MET	ILE	VAL	GLU	LEU
9	LEU	ASN	SER	ALA	ILE	GLU	ALA	VAL	VAL	ASP
10	ARG	ILE	GLY	SER	GLU	TYR	HIS	GLU	LEU	SER
11	GLY	ARG	ALA	LYS	ASP	MET	GLY	SER	ALA	ALA
12	VAL	LEU	ILE	ALA	ILE	ILE	ASP	ALA	VAL	ILE
13	THR	TRP	CYS	ILE	LEU	LEU	TRP	SER	HIS	PHE

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	GLU	LEU
2	ALA	ASN	ASN	THR	THR	GLY	PHE	THR	ARG	ILE
3	ILE	LYS	ALA	ALA	GLY	TYR	SER	TRP	LYS	GLY
4	LEU	ARG	ALA	ALA	TRP	ILE	ASN	GLU	ALA	ALA
5	PHE	ARG	GLN	GLU	GLY	VAL	ALA	VAL	LEU	LEU
6	ALA	VAL	VAL	ILE	ALA	CYS	TRP	LEU	ASP	VAL
7	ASP	ALA	CYS	THR	ARG	VAL	LEU	LEU	ILE	SER
8	SER	VAL	MET	LEU	VAL	MET	ILE	VAL	GLU	LEU
9	LEU	ASN	SER	ALA	ILE	GLU	ALA	VAL	VAL	ASP
10	ARG	ILE	GLY	SER	GLU	TYR	HIS	GLU	LEU	SER
11	GLY	ARG	ALA	LYS	ASP	MET	GLY	SER	ALA	ALA
12	VAL	LEU	ILE	ALA	ILE	ILE	ASP	ALA	VAL	ILE
13	THR	TRP	CYS	ILE	LEU	LEU	TRP	SER	HIS	PHE