BMRB Entry 19751

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PDB ID: 2mk4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19751
MolProbity Validation Chart

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Title:
Solution structure of ORF2
Deposition date:
2014-01-24
Original release date:
2015-03-23
Authors:
Miyakawa, Takuya; Kobayashi, Hidetomo; Tashiro, Mitsuru; Yamanaka, Hiroyasu; Tanokura, Masaru
Citation:

Citation: Kobayashi, Hidetomo; Yoshida, Toru; Miyakawa, Takuya; Tashiro, Mitsuru; Okamoto, Keinosuke; Yamanaka, Hiroyasu; Tanokura, Masaru; Tsuge, Hideaki. "Structural Basis for Action of the External Chaperone for a Propeptide-deficient Serine Protease from Aeromonas sobria"  J. Biol. Chem. ., .-. (2015).
PubMed: 25784551

Assembly members:

Assembly members:
entity, polymer, 133 residues, 13751.540 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 646   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Aeromonas sobria

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPVQESVTMDGKQYSTIEVN GQTYLIPDNGSKKRVARSLD SKVPQQTLRRGDVLMQGAAS PELTVSGTLLVEADDASAKA LATRHGLNFKQSSGGIALLE AKPGTDLNAIATKLKSEGVN VQIELSGAEQQPK

Data sets:
Data typeCount
13C chemical shifts416
15N chemical shifts119
1H chemical shifts794

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ORF21

Entities:

Entity 1, ORF2 133 residues - 13751.540 Da.

1   GLYPROVALGLNGLUSERVALTHRMETASP
2   GLYLYSGLNTYRSERTHRILEGLUVALASN
3   GLYGLNTHRTYRLEUILEPROASPASNGLY
4   SERLYSLYSARGVALALAARGSERLEUASP
5   SERLYSVALPROGLNGLNTHRLEUARGARG
6   GLYASPVALLEUMETGLNGLYALAALASER
7   PROGLULEUTHRVALSERGLYTHRLEULEU
8   VALGLUALAASPASPALASERALALYSALA
9   LEUALATHRARGHISGLYLEUASNPHELYS
10   GLNSERSERGLYGLYILEALALEULEUGLU
11   ALALYSPROGLYTHRASPLEUASNALAILE
12   ALATHRLYSLEULYSSERGLUGLYVALASN
13   VALGLNILEGLULEUSERGLYALAGLUGLN
14   GLNPROLYS

Related Database Links:

PDB 2MK4 3WQB
GB AEB49524 AHH32619 EKB12965 EKB17107 EKB21458
REF WP_005333882 WP_005352955 WP_005361466 WP_019445376 WP_021229060

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