BMRB Entry 19736

Name: 1H, 13C, and 15N Chemical Shift Assignments of a the mature form of Trypanosoma brucei 1-C-Grx1
Published: 2014-11-07

Click here to enlarge.

PDB ID: 2mxn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19736
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C, and 15N Chemical Shift Assignments of a the mature form of Trypanosoma brucei 1-C-Grx1

Deposition date:

2014-01-15

Original release date:

2014-11-07

Authors:

Sturlese, Mattia; Lelli, Moreno; Bellanda, Massimo; Manta, Bruno; Comini, Marcelo

Citation:

Citation: Sturlese, Mattia; Lelli, Moreno; Manta, Bruno; Mammi, Stefano; Comini, Marcelo; Bellanda, Massimo. "1H, 13C and 15N resonance assignment of the mature form of monothiol glutaredoxin 1 from the pathogen Trypanosoma brucei" Biomol. NMR Assignments ., .-. (2014).
PubMed: 24830542

Assembly members:

Assembly members:
1-C-Grx1, polymer, 145 residues, 16063.5 Da.

Natural source:

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET Trx-1b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
1-C-Grx1: GAMSTSGIGGDVRDIEETHP DFQPRLVSADLAEDEIAMVK KDIDDTIKSEDVVTFIKGLP EAPMCAYSKRMIDVLEALGL EYTSFDVLAHPVVRSYVKEV SEWPTIPQLFIKAEFVGGLD IVTKMLESGDLKKMLRDKGI TCRDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	594
15N chemical shifts	132
1H chemical shifts	869

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	1-C-Grx1	1

Entities:

Entity 1, 1-C-Grx1 145 residues - 16063.5 Da.

Residues 1-3 represent the residual cleavage site sequence.

1

GLY

ALA

MET

SER

THR

SER

GLY

ILE

GLY

2

ASP

VAL

ARG

ASP

ILE

GLU

THR

HIS

PRO

3

ASP

PHE

GLN

PRO

ARG

LEU

VAL

SER

ALA

ASP

4

LEU

ALA

GLU

ASP

GLU

ILE

ALA

MET

VAL

LYS

5

LYS

ASP

ILE

ASP

THR

ILE

LYS

SER

GLU

6

ASP

VAL

THR

PHE

ILE

LYS

GLY

LEU

PRO

7

GLU

ALA

PRO

MET

CYS

ALA

TYR

SER

LYS

ARG

8

MET

ILE

ASP

VAL

LEU

GLU

ALA

LEU

GLY

LEU

9

GLU

TYR

THR

SER

PHE

ASP

VAL

LEU

ALA

HIS

10

PRO

VAL

ARG

SER

TYR

VAL

LYS

GLU

VAL

11

SER

GLU

TRP

PRO

THR

ILE

PRO

GLN

LEU

PHE

12

ILE

LYS

ALA

GLU

PHE

VAL

GLY

LEU

ASP

13

ILE

VAL

THR

LYS

MET

LEU

GLU

SER

GLY

ASP

14

LEU

LYS

MET

LEU

ARG

ASP

LYS

GLY

ILE

15

THR

CYS

ARG

ASP

LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

EMBL	AJ619696.1 CAF02300 CBH14109
UNIPROT	Q2UZM9
NCBI	CAF02300
BMRB	18485
PDB	2LTK
GB	EAN76448
REF	XP_011776380 XP_803662
AlphaFold	Q2UZM9

BMRB Entry 19736

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: