BMRB Entry 19726
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PDB ID: 2mjh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19726
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Daubner, Gerrit; Bruemmer, Anneke; Tocchini, Cristina; Gerhardy, Stefan; Ciosk, Rafal; Zavolan, Mihaela; Allain, Frederic. "Structural and functional implications of the QUA2 domain on RNA recognition by GLD-1" .
Assembly members:
GermLine_development_Defective_1, polymer, 142 residues, 16075.667 Da.
5'-CUACUCAUAU-3', polymer, 10 residues, 3082.892 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTYB1
Entity Sequences (FASTA):
GermLine_development_Defective_1: LPEPAGDMISITEKIYVPKN
EYPDYNFVGRILGPRGMTAK
QLEQDTGCKIMVRGKGSMRD
KSKESAHRGKANWEHLEDDL
HVLVQCEDTENRVHIKLQAA
LEQVKKLLIPAPEGTDELKR
KQLMELAIINGTYRPMKSPN
PA
5'-CUACUCAUAU-3': CUACUCAUAU
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 13C chemical shifts | 386 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 1010 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GLD-1 KH-QUA2 bound to 5'-CUACUCAUAU-3' | 1 |
| 2 | RNA | 2 |
Entities:
Entity 1, GLD-1 KH-QUA2 bound to 5'-CUACUCAUAU-3' 142 residues - 16075.667 Da.
| 1 | LEU | PRO | GLU | PRO | ALA | GLY | ASP | MET | ILE | SER | ||||
| 2 | ILE | THR | GLU | LYS | ILE | TYR | VAL | PRO | LYS | ASN | ||||
| 3 | GLU | TYR | PRO | ASP | TYR | ASN | PHE | VAL | GLY | ARG | ||||
| 4 | ILE | LEU | GLY | PRO | ARG | GLY | MET | THR | ALA | LYS | ||||
| 5 | GLN | LEU | GLU | GLN | ASP | THR | GLY | CYS | LYS | ILE | ||||
| 6 | MET | VAL | ARG | GLY | LYS | GLY | SER | MET | ARG | ASP | ||||
| 7 | LYS | SER | LYS | GLU | SER | ALA | HIS | ARG | GLY | LYS | ||||
| 8 | ALA | ASN | TRP | GLU | HIS | LEU | GLU | ASP | ASP | LEU | ||||
| 9 | HIS | VAL | LEU | VAL | GLN | CYS | GLU | ASP | THR | GLU | ||||
| 10 | ASN | ARG | VAL | HIS | ILE | LYS | LEU | GLN | ALA | ALA | ||||
| 11 | LEU | GLU | GLN | VAL | LYS | LYS | LEU | LEU | ILE | PRO | ||||
| 12 | ALA | PRO | GLU | GLY | THR | ASP | GLU | LEU | LYS | ARG | ||||
| 13 | LYS | GLN | LEU | MET | GLU | LEU | ALA | ILE | ILE | ASN | ||||
| 14 | GLY | THR | TYR | ARG | PRO | MET | LYS | SER | PRO | ASN | ||||
| 15 | PRO | ALA |
Entity 2, RNA 10 residues - 3082.892 Da.
| 1 | C | U | A | C | U | C | A | U | A | U |
Related Database Links:
| PDB | 2MJH 4JVY |
| EMBL | CAB03417 |
| GB | AAC46632 EFP08647 |
| PRF | 2116296A |
| REF | NP_492143 XP_003100676 |
| SP | Q17339 |
| AlphaFold | Q17339 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks