BMRB Entry 19720

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19720

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Title:
NMR backbone assignment of WT apo-HasAp from Pseudomonas aeruginosa
Deposition date:
2014-01-07
Original release date:
2014-04-11
Authors:
Yao, Huili; Rivera, Mario
Citation:

Citation: Kumar, Ritesh; Matsumura, Hirotoshi; Lovell, Scott; Yao, Huili; Rodriguez, Juan; Battaile, Kevin; Moenne-Loccoz, Pierre; Rivera, Mario. "Replacing the Axial Ligand Tyrosine 75 or Its Hydrogen Bond Partner Histidine 83 Minimally Affects Hemin Acquisition by the Hemophore HasAp from Pseudomonas aeruginosa."  Biochemistry 53, 2112-2125 (2014).
PubMed: 24625274

Assembly members:

Assembly members:
WT_apo-HasAp, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WT_apo-HasAp: MSISISYSTTYSGWTVADYL ADWSAYFGDVNHRPGQVVDG SNTGGFNPGPFDGSQYALKS TASDAAFIAGGDLHYTLFSN PSHTLWGKLDSIALGDTLTG GASSGGYALDSQEVSFSNLG LDSPIAQGRDGTVHKVVYGL MSGDSSALQGQIDALLKAVD PSLSINSTFDQLAAAGVAHA TPAA

Data sets:
Data typeCount
13C chemical shifts173
15N chemical shifts164
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1WT apo HasAp1

Entities:

Entity 1, WT apo HasAp 184 residues - Formula weight is not available

1   METSERILESERILESERTYRSERTHRTHR
2   TYRSERGLYTRPTHRVALALAASPTYRLEU
3   ALAASPTRPSERALATYRPHEGLYASPVAL
4   ASNHISARGPROGLYGLNVALVALASPGLY
5   SERASNTHRGLYGLYPHEASNPROGLYPRO
6   PHEASPGLYSERGLNTYRALALEULYSSER
7   THRALASERASPALAALAPHEILEALAGLY
8   GLYASPLEUHISTYRTHRLEUPHESERASN
9   PROSERHISTHRLEUTRPGLYLYSLEUASP
10   SERILEALALEUGLYASPTHRLEUTHRGLY
11   GLYALASERSERGLYGLYTYRALALEUASP
12   SERGLNGLUVALSERPHESERASNLEUGLY
13   LEUASPSERPROILEALAGLNGLYARGASP
14   GLYTHRVALHISLYSVALVALTYRGLYLEU
15   METSERGLYASPSERSERALALEUGLNGLY
16   GLNILEASPALALEULEULYSALAVALASP
17   PROSERLEUSERILEASNSERTHRPHEASP
18   GLNLEUALAALAALAGLYVALALAHISALA
19   THRPROALAALA

Related Database Links:

BMRB 15962 15963 19721 19722
PDB 3ELL 3MOK 3MOL 3MOM 3W8M 3W8O 3WAH 4O6Q 4O6S 4O6T 4O6U
DBJ BAK91367 BAP21966 BAP49596 BAQ38483 BAR66548
EMBL CAA76520 CAW26382 CCQ88896 CDH69922 CDH76002
GB AAG06795 AAT49927 ABJ12662 ABR84528 AEO74046
REF NP_252097 WP_003091842 WP_003115011 WP_003124283 WP_003153113

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks