BMRB Entry 19703

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments and structure of Iron-sulfur cluster binding protein from Ehrlichia chaffeensis
Published: 2014-01-14

Click here to enlarge.

PDB ID: 2mj3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19703
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments and structure of Iron-sulfur cluster binding protein from Ehrlichia chaffeensis

Deposition date:

2013-12-23

Original release date:

2014-01-14

Authors:

Barnwal, Ravi Pratap; Varani, Gabriele

Citation:

Citation: Barnwal, Ravi Pratap; Varani, Gabriele. "Backbone 1H, 13C, and 15N Chemical Shift Assignments and structure of Iron-sulfur cluster binding protein from Ehrlichia chaffeensis" .

Assembly members:

Assembly members:
Iron-sulfur_cluster_binding_protein, polymer, 116 residues, 12862.470 Da.

Natural source:

Natural source: Common Name: Ehrlichia chaffeensis Taxonomy ID: 945 Superkingdom: Bacteria Kingdom: not available Genus/species: Ehrlichia chaffeensis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-AVA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Iron-sulfur_cluster_binding_protein: MPLITFISPDGSRKTYEAYD GETLLSLAHRNNVDLEGACE GSLACSTCHVIIDPSWYDIV EQHNEISDEENDMLDLAFGL TDTSRLGCQIILTKELDGLC VILPTETRNISFVKNS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	393
15N chemical shifts	98
1H chemical shifts	563

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Iron-sulfur cluster binding protein	1

Entities:

Entity 1, Iron-sulfur cluster binding protein 116 residues - 12862.470 Da.

1

MET

PRO

LEU

ILE

THR

PHE

ILE

SER

PRO

ASP

2

GLY

SER

ARG

LYS

THR

TYR

GLU

ALA

TYR

ASP

3

GLY

GLU

THR

LEU

SER

LEU

ALA

HIS

ARG

4

ASN

VAL

ASP

LEU

GLU

GLY

ALA

CYS

GLU

5

GLY

SER

LEU

ALA

CYS

SER

THR

CYS

HIS

VAL

6

ILE

ASP

PRO

SER

TRP

TYR

ASP

ILE

VAL

7

GLU

GLN

HIS

ASN

GLU

ILE

SER

ASP

GLU

8

ASN

ASP

MET

LEU

ASP

LEU

ALA

PHE

GLY

LEU

9

THR

ASP

THR

SER

ARG

LEU

GLY

CYS

GLN

ILE

10

ILE

LEU

THR

LYS

GLU

LEU

ASP

GLY

LEU

CYS

11

VAL

ILE

LEU

PRO

THR

GLU

THR

ARG

ASN

ILE

12

SER

PHE

VAL

LYS

ASN

SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

PDB	2MJ3
EMBL	CEI85472
GB	AAZ68448 ABD45498 AHX03715 AHX04894 AHX05564
REF	WP_006011527 WP_011304526 WP_044194855 WP_045171525

BMRB Entry 19703

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: