BMRB Entry 19682

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PDB ID: 2miq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19682
MolProbity Validation Chart

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Title:
Solution NMR Structure of PHD Type 1 Zinc Finger Domain 1 of Lysine-specific Demethylase Lid from Drosophila melanogaster, Northeast Structural Genomics Consortium (NESG) Target FR824J
Deposition date:
2013-12-13
Original release date:
2014-01-22
Authors:
Xu, Xianzhong; Eletsky, Alexander; Lee, Dan; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Sukumaran, Dinesh; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Xu, Xianzhong; Eletsky, Alexander; Lee, Dan; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Sukumaran, Dinesh; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR Structure of PHD Type 1 Zinc Finger Domain 1 from Lysine-specific Demethylase Lid from Drosophila melanogaster, Northeast Structural Genomics Consortium (NESG) Target FR824J"  To be published ., .-..

Assembly members:

Assembly members:
FR824J, polymer, 94 residues, 10196.379 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15Avi6Ht_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FR824J: SHMSGGSPLATGTTANTRGA SQKKGGEPPALIVDPLMKYI CHICNRGDVEESMLLCDGCD DSYHTFCLLPPLTSIPKGEW LCPRCVVEEVSKPQ

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts95
1H chemical shifts628

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FR824J1
2Zn-12
3Zn-22

Entities:

Entity 1, FR824J 94 residues - 10196.379 Da.

Residues 1-3 represent a non-native affinity tag remainder after his tag cleavage.

1   SERHISMETSERGLYGLYSERPROLEUALA
2   THRGLYTHRTHRALAASNTHRARGGLYALA
3   SERGLNLYSLYSGLYGLYGLUPROPROALA
4   LEUILEVALASPPROLEUMETLYSTYRILE
5   CYSHISILECYSASNARGGLYASPVALGLU
6   GLUSERMETLEULEUCYSASPGLYCYSASP
7   ASPSERTYRHISTHRPHECYSLEULEUPRO
8   PROLEUTHRSERILEPROLYSGLYGLUTRP
9   LEUCYSPROARGCYSVALVALGLUGLUVAL
10   SERLYSPROGLN

Entity 2, Zn-1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

UNP Q9VMJ7
PDB 2MIQ
GB AAF52319 AAM11379 AAN10569 AFH03582 AFH03583
REF NP_001245908 NP_001245909 NP_001245910 NP_001285649 NP_523486
SP Q9VMJ7
AlphaFold Q9VMJ7 Q9VMJ7

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