BMRB Entry 19633

Name: Solution structure of a EF-hand domain from sea urchin polycystin-2
Published: 2014-04-11

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PDB ID: 2mhh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19633
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of a EF-hand domain from sea urchin polycystin-2

Deposition date:

2013-11-22

Original release date:

2014-04-11

Authors:

Kuo, Ivana; Keeler, Camille; Corbin, Rachel; Celic, Andjelka; Petri, Edward; Hodsdon, Michael; Ehrlich, Barbara

Citation:

Citation: Kuo, Ivana; Keeler, Camille; Corbin, Rachel; Celic, Andjelka; Petri, Edward; Hodsdon, Michael; Ehrlich, Barbara. "The number and location of EF hand motifs dictates the calcium dependence of polycystin-2 function." FASEB J. ., .-. (2014).
PubMed: 24558196

Assembly members:

Assembly members:
entity, polymer, 96 residues, 10744.843 Da.

Natural source:

Natural source: Common Name: purple sea urchin Taxonomy ID: 7668 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Strongylocentrotus purpuratus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GGLVPRGSHMASKRDKIADI QEALAHADANADQHLDFDEW RQELKCRGHADADIEAVFAK YDVDGDRVLDAEEQMKMAHD LEGQKSDLNNQLAELE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	386
15N chemical shifts	104
1H chemical shifts	596

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EF-hand domain from sea urchin polycystin-2	1

Entities:

Entity 1, EF-hand domain from sea urchin polycystin-2 96 residues - 10744.843 Da.

1

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

MET

2

ALA

SER

LYS

ARG

ASP

LYS

ILE

ALA

ASP

ILE

3

GLN

GLU

ALA

LEU

ALA

HIS

ALA

ASP

ALA

ASN

4

ALA

ASP

GLN

HIS

LEU

ASP

PHE

ASP

GLU

TRP

5

ARG

GLN

GLU

LEU

LYS

CYS

ARG

GLY

HIS

ALA

6

ASP

ALA

ASP

ILE

GLU

ALA

VAL

PHE

ALA

LYS

7

TYR

ASP

VAL

ASP

GLY

ASP

ARG

VAL

LEU

ASP

8

ALA

GLU

GLN

MET

LYS

MET

ALA

HIS

ASP

9

LEU

GLU

GLY

GLN

LYS

SER

ASP

LEU

ASN

10

GLN

LEU

ALA

GLU

LEU

GLU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	anisotropic	sample_conditions_1

PDB	2MHH
GB	AAP35006
REF	NP_999827 XP_011662382

BMRB Entry 19633

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: