BMRB Entry 19563

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19563

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Title:
chemical shift assignments for human dihdyrofolate reductase bound to folate
Deposition date:
2013-10-18
Original release date:
2016-04-04
Authors:
Wright, Peter; Bhabha, Gira; Tuttle, Lisa; Kroon, Gerard
Citation:

Citation: Tuttle, Lisa; Dyson, H. Jane; Wright, Peter. "Side Chain Conformational Averaging in Human Dihydrofolate Reductase"  Biochemistry 53, 1134-1145 (2014).
PubMed: 24498949

Assembly members:

Assembly members:
DHFR, polymer, 187 residues, Formula weight is not available
FOLIC ACID, non-polymer, 441.397 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DHFR: MVGSLNCIVAVSQNMGIGKN GDLPWPPLRNEFRYFQRMTT TSSVEGKQNLVIMGKKTWFS IPEKNRPLKGRINLVLSREL KEPPQGAHFLSRSLDDALKL TEQPELANKVDMVWIVGGSS VYKEAMNHPGHLKLFVTRIM QDFESDTFFPEIDLEKYKLL PEYPGVLSDVQEEKGIKYKF EVYEKND

Data sets:
Data typeCount
13C chemical shifts180
15N chemical shifts168
1H chemical shifts168

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DHFR1
2Folate2

Entities:

Entity 1, DHFR 187 residues - Formula weight is not available

1   METVALGLYSERLEUASNCYSILEVALALA
2   VALSERGLNASNMETGLYILEGLYLYSASN
3   GLYASPLEUPROTRPPROPROLEUARGASN
4   GLUPHEARGTYRPHEGLNARGMETTHRTHR
5   THRSERSERVALGLUGLYLYSGLNASNLEU
6   VALILEMETGLYLYSLYSTHRTRPPHESER
7   ILEPROGLULYSASNARGPROLEULYSGLY
8   ARGILEASNLEUVALLEUSERARGGLULEU
9   LYSGLUPROPROGLNGLYALAHISPHELEU
10   SERARGSERLEUASPASPALALEULYSLEU
11   THRGLUGLNPROGLULEUALAASNLYSVAL
12   ASPMETVALTRPILEVALGLYGLYSERSER
13   VALTYRLYSGLUALAMETASNHISPROGLY
14   HISLEULYSLEUPHEVALTHRARGILEMET
15   GLNASPPHEGLUSERASPTHRPHEPHEPRO
16   GLUILEASPLEUGLULYSTYRLYSLEULEU
17   PROGLUTYRPROGLYVALLEUSERASPVAL
18   GLNGLUGLULYSGLYILELYSTYRLYSPHE
19   GLUVALTYRGLULYSASNASP

Entity 2, Folate - 441.397 Da.

1   FOL

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