BMRB Entry 19554

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PDB ID: 2mfl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19554
MolProbity Validation Chart

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Title:
Domain 2 of E. coli ribosomal protein S1
Deposition date:
2013-10-12
Original release date:
2014-04-14
Authors:
GIRAUD, Pierre; CRECHET, Jean-Bernard; BONTEMS, Francois; UZAN, Marc; SIZUN, Christina
Citation:

Citation: Giraud, Pierre; Crechet, Jean-Bernard; Uzan, Marc; Bontems, Francois; Sizun, Christina. "Resonance assignment of the ribosome binding domain of E. coli ribosomal protein S1."  Biomol. NMR Assignments ., .-. (2014).
PubMed: 24682851

Assembly members:

Assembly members:
S1F12, polymer, 182 residues, 10462.053 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S1F12: GSHMTESFAQLFEESLKEIE TRPGSIVRGVVVAIDKDVVL VDAGLKSESAIPAEQFKNAQ GELEIQVGDEVDVALDAVED GFGETLLSREKAKRHEAWIT LEKAYEDAETVTGVINGKVK GGFTVELNGIRAFLPGSLVD VRPVRDTLHLEGKELEFKVI KLDQKRNNVVVSRRAVIESE NS

Data sets:
Data typeCount
13C chemical shifts668
15N chemical shifts174
1H chemical shifts1088

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S1F121

Entities:

Entity 1, S1F12 182 residues - 10462.053 Da.

This sequence contains the two first domains of S1 separated by a flexible linker and an extended flexible N-terminal region

1   GLYSERHISMETTHRGLUSERPHEALAGLN
2   LEUPHEGLUGLUSERLEULYSGLUILEGLU
3   THRARGPROGLYSERILEVALARGGLYVAL
4   VALVALALAILEASPLYSASPVALVALLEU
5   VALASPALAGLYLEULYSSERGLUSERALA
6   ILEPROALAGLUGLNPHELYSASNALAGLN
7   GLYGLULEUGLUILEGLNVALGLYASPGLU
8   VALASPVALALALEUASPALAVALGLUASP
9   GLYPHEGLYGLUTHRLEULEUSERARGGLU
10   LYSALALYSARGHISGLUALATRPILETHR
11   LEUGLULYSALATYRGLUASPALAGLUTHR
12   VALTHRGLYVALILEASNGLYLYSVALLYS
13   GLYGLYPHETHRVALGLULEUASNGLYILE
14   ARGALAPHELEUPROGLYSERLEUVALASP
15   VALARGPROVALARGASPTHRLEUHISLEU
16   GLUGLYLYSGLULEUGLUPHELYSVALILE
17   LYSLEUASPGLNLYSARGASNASNVALVAL
18   VALSERARGARGALAVALILEGLUSERGLU
19   ASNSER

Related Database Links:

UNP P0AG67
BMRB 19550
PDB 2MFI 2MFL 4Q7J 4R71
DBJ BAA35655 BAB34417 BAG76494 BAH62634 BAI24353
EMBL CAA23630 CAA23644 CAD05381 CAO97188 CAP75381
GB AAC73997 AAG55396 AAL19915 AAN42537 AAN79519
PIR AC0614
PRF 0804233A
REF NP_309021 NP_415431 NP_455468 NP_459956 NP_706830
SP P0AG67 P0AG68 P0AG69 P0AG70
AlphaFold P77352 P0AG67 P0AG68 P0AG69 P0AG70

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks