BMRB Entry 19524

Title:
NMR solution structure of the GS-TAMAPIN MUTATION R7A.
Deposition date:
2013-09-24
Original release date:
2014-05-27
Authors:
del Rio-Portilla, F.; Ramirez-Cordero, B.
Citation:

Citation: Ramirez-Cordero, Belen; Toledano, Yanis; Cano-Sanchez, Patricia; Hernandez-Lopez, Rogelio; Flores-Solis, David; Saucedo-Yanez, Alma; Chavez-Uribe, Isabel; Brieba, Luis; Del Rio-Portilla, Federico. "Cytotoxicity of recombinant tamapin and related toxin-like peptides on model cell lines."  Chem. Res. Toxicol. 27, 960-967 (2014).
PubMed: 24821061

Assembly members:

Assembly members:
entity, polymer, 32 residues, 3528.202 Da.

Natural source:

Natural source:   Common Name: scorpions   Taxonomy ID: 34647   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus tamulus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pEt32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SAFCNLRACELSCRSLGLLG KCIGEECKCVPY

Data sets:
Data typeCount
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GS-TAMAPIN MUTATION R7A1

Entities:

Entity 1, GS-TAMAPIN MUTATION R7A 32 residues - 3528.202 Da.

1   SERALAPHECYSASNLEUARGALACYSGLU
2   LEUSERCYSARGSERLEUGLYLEULEUGLY
3   LYSCYSILEGLYGLUGLUCYSLYSCYSVAL
4   PROTYR

Samples:

sample_1: entity 2.5 ± 0.1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 3.35; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v99, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz