BMRB Entry 19520

Click here to enlarge.

PDB ID: 2me8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19520
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution Structure of BCL-xL in its p53-bound conformation determined with selective isotope labelling of I,L,V sidechains
Deposition date:
2013-09-25
Original release date:
2014-04-28
Authors:
Viacava Follis, Ariele; Grace, Christy; Kriwacki, Richard
Citation:

Citation: Viacava Follis, Ariele; Ou, Li; Llambi, Fabien; Green, Douglas; Kriwacki, Richard. "The DNA Binding Domain is a Promiscuous Interaction Hub that Mediates the Nuclear and Cytoplasmic Functions of p53"  Nat. Struct. Biol. ., .-..

Assembly members:

Assembly members:
BCL-xL, polymer, 212 residues, 23670.139 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BCL-xL: GHSMSQSNRELVVDFLSYKL SQKGYSWSQFSDVEENRTEA PEGTESEMETPSAINGNPSW HLADSPAVNGATGHSSSLDA REVIPMAAVKQALREAGDEF ELRYRRAFSDLTSQLHITPG TAYQSFEQVVNELFRDGVNW GRIVAFFSFGGALCVESVDK EMQVLVSRIAAWMATYLNDH LEPWIQENGGWDTFVELYGN NAAAESRKGQER

Data sets:
Data typeCount
13C chemical shifts646
15N chemical shifts209
1H chemical shifts399

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BCL-xL1

Entities:

Entity 1, BCL-xL 212 residues - 23670.139 Da.

1   GLYHISSERMETSERGLNSERASNARGGLU
2   LEUVALVALASPPHELEUSERTYRLYSLEU
3   SERGLNLYSGLYTYRSERTRPSERGLNPHE
4   SERASPVALGLUGLUASNARGTHRGLUALA
5   PROGLUGLYTHRGLUSERGLUMETGLUTHR
6   PROSERALAILEASNGLYASNPROSERTRP
7   HISLEUALAASPSERPROALAVALASNGLY
8   ALATHRGLYHISSERSERSERLEUASPALA
9   ARGGLUVALILEPROMETALAALAVALLYS
10   GLNALALEUARGGLUALAGLYASPGLUPHE
11   GLULEUARGTYRARGARGALAPHESERASP
12   LEUTHRSERGLNLEUHISILETHRPROGLY
13   THRALATYRGLNSERPHEGLUGLNVALVAL
14   ASNGLULEUPHEARGASPGLYVALASNTRP
15   GLYARGILEVALALAPHEPHESERPHEGLY
16   GLYALALEUCYSVALGLUSERVALASPLYS
17   GLUMETGLNVALLEUVALSERARGILEALA
18   ALATRPMETALATHRTYRLEUASNASPHIS
19   LEUGLUPROTRPILEGLNGLUASNGLYGLY
20   TRPASPTHRPHEVALGLULEUTYRGLYASN
21   ASNALAALAALAGLUSERARGLYSGLYGLN
22   GLUARG

Related Database Links:

GB CAA80661 AAA51039 AAA82173 AAA82174 AAB17352 AAB17353
BMRB 19521 19522
PDB 1AF3 1BXL 1LXL 1MAZ 1PQ0 1PQ1 1R2D 1R2E 1R2G 1R2H 1R2I 2B48 2BZW 2ME8 2ME9 2MEJ 3CVA 3IHC 3IHD 3IHE 3IHF 3IIG 3IIH 3ILB 3ILC 4HNJ
DBJ BAB71819 BAB85856 BAE27189 BAE42906 BAE73044
EMBL CAA04597 CAA57886 CAA58557 CAA80661 CAI56777
REF NP_001003072 NP_001009226 NP_001009228 NP_001028842 NP_001028844
SP O77737 P53563 Q07817 Q64373
TPG DAA23121
AlphaFold O77737 P53563 Q07817 Q64373

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks