BMRB Entry 19501

Title:
A novel 4/7-conotoxin LvIA from Conus lividus that selectively blocks 32 vs. 6/323 nicotinic acetylcholine receptors
Deposition date:
2013-09-16
Original release date:
2014-02-10
Authors:
Schroeder, Christina
Citation:

Citation: Luo, Sulan; Zhangsun, Dongting; Schroeder, Christina; Zhu, Xiaopeng; Hu, Yuanyan; Wu, Yong; Weltzin, Maegan; Eberhard, Spencer; Kaas, Quentin; Craik, David; McIntosh, J. Michael; Whiteaker, Paul. "A novel 4/7-conotoxin LvIA from Conus lividus that selectively blocks 32 vs. 6/323 nicotinic acetylcholine receptors."  FASEB J. 28, 1842-1853 (2014).
PubMed: 24398291

Assembly members:

Assembly members:
entity, polymer, 16 residues, 1686.907 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GCCSHPACNVDHPEIC

Data sets:
Data typeCount
13C chemical shifts40
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Novel 4/7-Conotoxin LvIA1

Entities:

Entity 1, Novel 4/7-Conotoxin LvIA 16 residues - 1686.907 Da.

1   GLYCYSCYSSERHISPROALACYSASNVAL
2   ASPHISPROGLUILECYS

Samples:

sample_1: Novel 4/7-Conotoxin LvIA 1.5 mM; H2O 90%; D2O 10%

sample_2: Novel 4/7-Conotoxin LvIA 1.5 mM; D2O 100%

sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 297.5 K

sample_conditions_2: pH: 4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

XEASY, Bartels et al. - chemical shift assignment, chemical shift calculation, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CCP46959
GB AAD31913
SP L8BU87 Q9XZK7
AlphaFold L8BU87 Q9XZK7