BMRB Entry 19501
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19501
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Luo, Sulan; Zhangsun, Dongting; Schroeder, Christina; Zhu, Xiaopeng; Hu, Yuanyan; Wu, Yong; Weltzin, Maegan; Eberhard, Spencer; Kaas, Quentin; Craik, David; McIntosh, J. Michael; Whiteaker, Paul. "A novel 4/7-conotoxin LvIA from Conus lividus that selectively blocks 32 vs. 6/323 nicotinic acetylcholine receptors." FASEB J. 28, 1842-1853 (2014).
PubMed: 24398291
Assembly members:
entity, polymer, 16 residues, 1686.907 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: GCCSHPACNVDHPEIC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 40 |
| 1H chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Novel 4/7-Conotoxin LvIA | 1 |
Entities:
Entity 1, Novel 4/7-Conotoxin LvIA 16 residues - 1686.907 Da.
| 1 | GLY | CYS | CYS | SER | HIS | PRO | ALA | CYS | ASN | VAL | ||||
| 2 | ASP | HIS | PRO | GLU | ILE | CYS |
Samples:
sample_1: Novel 4/7-Conotoxin LvIA 1.5 mM; H2O 90%; D2O 10%
sample_2: Novel 4/7-Conotoxin LvIA 1.5 mM; D2O 100%
sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 297.5 K
sample_conditions_2: pH: 4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
XEASY, Bartels et al. - chemical shift assignment, chemical shift calculation, peak picking
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
TOPSPIN, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance 600 MHz