BMRB Entry 19488

Title:
Solution structure of WW domain with polyproline stretch (PP2WW) of HYPB
Deposition date:
2013-09-11
Original release date:
2014-03-21
Authors:
Gao, Yong-Guang; Hu, Hong-Yu
Citation:

Citation: Gao, Yong-Guang; Yang, Hui; Zhao, Jian; Jiang, Ya-Jun; Hu, Hong-Yu. "Autoinhibitory Structure of the WW Domain of HYPB/SETD2 Regulates Its Interaction with the Proline-Rich Region of Huntingtin"  Structure 22, 378-386 (2014).
PubMed: 24412394

Assembly members:

Assembly members:
entity, polymer, 56 residues, 6336.088 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32M

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts150
15N chemical shifts46
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PP2WW of HYPB1

Entities:

Entity 1, PP2WW of HYPB 56 residues - 6336.088 Da.

1   GLYSERASPLEUPROPROPROSERPROPRO
2   LYSPROLYSTHRILEVALLEUPROPROASN
3   TRPLYSTHRALAARGASPPROGLUGLYLYS
4   ILETYRTYRTYRHISVALILETHRARGGLN
5   THRGLNTRPASPPROPROTHRTRPGLUSER
6   PROGLYASPASPALASER

Samples:

sample_1: PP2WW, [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.05 w/v %

sample_conditions_1: ionic strength: 0.08 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

ARIA, Linge, O'Donoghue and Nilges - structure solution

Molmol, Koradi, Billeter and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19483
PDB
DBJ BAB21823 BAD32524 BAG10485
EMBL CAC28349 CAD28492
GB AAC26194 AAC26846 AAH31601 AAH59049 AAH90954
REF NP_001074809 NP_001101659 NP_054878 XP_001113652 XP_001495700
SP E9Q5F9 Q9BYW2
TPG DAA16805
AlphaFold E9Q5F9 Q9BYW2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks