BMRB Entry 19482

Assembly:

Entities:

Entity 1, Regulatory Domain of Tyrosine Hydroxylase 93 residues - 10485.973 Da.

Samples:

sample_1: RDTyrH65-159, [U-95% 15N], 0.8 mM; sodium phosphate 50 mM; D2O, [U-100% 2H], 5%; H2O, [U-100% 2H], 95%

sample_2: RDTyrH65-159, [U-95% 13C; U-95% 15N], 1.0-1.2 mM; sodium phosphate 50 mM; D2O, [U-100% 2H], 5%; h2O, [U-100% 2H], 95%

sample_3: RDTyrH65-159 0.9 mM; RDTyrH65-159, [U-2H; U-15N], 0.9 mM; sodium phosphate 50 mM; D2O, [U-100% 2H], 5%; H2O, [U-100% 2H], 95%

sample_4: RDTyrH65-159, [U-95% 15N], 0.8 mM; sodium phosphate 50 mM; D2O, [U-100% 2H], 5%; Pf1 phage 8 mg/mL; H2O, [U-100% 2H], 95%

sample_conditions_1: ionic strength: 0.11 M; pH: 7; pressure: 1 atm; temperature: 300 K

Experiments:

Software:

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

ModelFree, Palmer - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

PECAN, Eghbalnia, Wang, Bahrami, Assadi, and Markley - chemical shift calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ProcheckNMR, Laskowski and MacArthur - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

• Bruker Avance 700 MHz
• Bruker Avance 600 MHz
• Bruker Avance 500 MHz