BMRB Entry 19398

Name: Solution structure of the forkhead domain of Brugia malayi DAF-16a
Published: 2013-09-10

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PDB ID: 2mbf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19398
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the forkhead domain of Brugia malayi DAF-16a

Deposition date:

2013-07-30

Original release date:

2013-09-10

Authors:

Veldkamp, C.; Peterson, F.; Casper, S.; Schoeller, S.

Citation:

Citation: Veldkamp, C.; Peterson, F.; Casper, S.; Schoeller, S.. "Solution structure of the forkhead domain of Brugia malayi DAF-16a" To be published ., .-..

Assembly members:

Assembly members:
Bm-DAF-16a, polymer, 101 residues, 11673.083 Da.

Natural source:

Natural source: Common Name: nematodes Taxonomy ID: 6279 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Brugia malayi

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bm-DAF-16a: NPWGAESYSDLIAKALKSTF DGRMRLNEIYNWFASNVPYF GNRTSQEQSAGWKNSIRHNL SLHSRFMRIQNEGAGKSSWW VINPDAKPGRNPRRQRSATL E

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	423
15N chemical shifts	109
1H chemical shifts	687

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Forkhead DNA binding domain of Brugia malayi DAF-16a	1

Entities:

Entity 1, Forkhead DNA binding domain of Brugia malayi DAF-16a 101 residues - 11673.083 Da.

1

ASN

PRO

TRP

GLY

ALA

GLU

SER

TYR

SER

ASP

2

LEU

ILE

ALA

LYS

ALA

LEU

LYS

SER

THR

PHE

3

ASP

GLY

ARG

MET

ARG

LEU

ASN

GLU

ILE

TYR

4

ASN

TRP

PHE

ALA

SER

ASN

VAL

PRO

TYR

PHE

5

GLY

ASN

ARG

THR

SER

GLN

GLU

GLN

SER

ALA

6

GLY

TRP

LYS

ASN

SER

ILE

ARG

HIS

ASN

LEU

7

SER

LEU

HIS

SER

ARG

PHE

MET

ARG

ILE

GLN

8

ASN

GLU

GLY

ALA

GLY

LYS

SER

TRP

9

VAL

ILE

ASN

PRO

ASP

ALA

LYS

PRO

GLY

ARG

10

ASN

PRO

ARG

GLN

ARG

SER

ALA

THR

LEU

11

GLU

Samples:

sample: Forkhead box DNA binding domain of Brugia malayi DAF-16a, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 90%; D20 10%; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	sample	isotropic	sample_conditions_1
3D_13C-separated_NOESY	sample	isotropic	sample_conditions_1
3D_13C-separated_NOESY (AROMATIC)	sample	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CARA v1.8.4, Keller, R. - data analysis

CYANA v3.0, Guntert, P. - structural calculation

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2MBF
EMBL	CDQ02878
GB	EJW78700
REF	XP_001901487