BMRB Entry 19342

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19342
MolProbity Validation Chart

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Title:
Transmembrane-cytosolic part of Trop2 explored by NMR and Molecular Dynamics
Deposition date:
2013-07-08
Original release date:
2014-07-07
Authors:
Vidmar, Tilen; Pav i, Miha; Ilc, Gregor; Plavec, Janez; Lenar i, Brigita
Citation:

Citation: Vidmar, Tilen; Pav i, Miha; Ilc, Gregor; Plavec, Janez; Lenar i, Brigita. "Transmembrane-cytosolic part of Trop2 explored by NMR and Molecular Dynamics"  .

Assembly members:

Assembly members:
entity, polymer, 26 residues, 3096.678 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: TNRRKSGKYKKVEIKELGEL RKEPSL

Data sets:
Data typeCount
13C chemical shifts79
15N chemical shifts25
1H chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Transmembrane-cytosolic part of Trop21

Entities:

Entity 1, Transmembrane-cytosolic part of Trop2 26 residues - 3096.678 Da.

1   THRASNARGARGLYSSERGLYLYSTYRLYS
2   LYSVALGLUILELYSGLULEUGLYGLULEU
3   ARGLYSGLUPROSERLEU

Samples:

sample_1: entity mM; DSS 5.5 mM; TFE 70%; H2O 30%

sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

YASARA, Krieger E. - refinement

NMR spectrometers:

  • Varian VNMRS 900 MHz

Related Database Links:

BMRB 25274
PDB
EMBL CAA31781 CAA54799 CAA54801 CAG47056
GB AAA52505 AAH09409 AAP35919 AAP88905 AAX29730
REF NP_002344 XP_002810812 XP_003265191 XP_003824268 XP_003892008
SP P09758
AlphaFold P09758

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks