BMRB Entry 19319

Title:
Solution structure of RasGRP2 EF hands bound to calcium
Deposition date:
2013-06-24
Original release date:
2013-08-26
Authors:
Kuriyan, John; Iwig, Jeffrey; Vercoulen, Yvonne; Das, Rahul; Barros, Tiago; Limnander, Andre; Che, Yan; Pelton, Jeffrey; Wemmer, David; Roose, Jeroen
Citation:

Citation: Iwig, Jeffrey; Vercoulen, Yvonne; Das, Rahul; Barros, Tiago; Limnander, Andre; Che, Yan; Pelton, Jeffrey; Wemmer, David; Roose, Jeroen; Kuriyan, John. "Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1"  eLife 2, e00813-e00813 (2013).
PubMed: 23908768

Assembly members:

Assembly members:
RasGRP2, polymer, 81 residues, 18412.633 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSMT3

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts304
15N chemical shifts77
1H chemical shifts386

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RasGRP2 EF hands1
2calcium ion2

Entities:

Entity 1, RasGRP2 EF hands 81 residues - 18412.633 Da.

1   LYSLEUASPGLNALALEUVALVALGLUHIS
2   ILEGLULYSMETVALGLUSERVALPHEARG
3   ASNPHEASPVALASPGLYASPGLYHISILE
4   SERGLNGLUGLUPHEGLNILEILEARGGLY
5   ASNPHEPROTYRLEUSERALAPHEGLYASP
6   LEUASPGLNASNGLNASPGLYCYSILESER
7   ARGGLUGLUMETVALSERTYRPHELEUARG
8   SERSERSERVALLEUGLYGLYARGMETGLY
9   PHE

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: RasGRP2, [U-99% 13C; U-99% 15N], 500 uM; HEPES 25 mM; sodium chloride 100 mM; TCEP 1 mM; D2O 7%; H2O 93%

sample_2: RasGRP2, [U-99% 13C; U-99% 15N], 500 uM; HEPES 25 mM; sodium chloride 100 mM; TCEP 1 mM; D2O 7%; H2O 93%; Pf1 phage 17 mg/mL

sample_conditions_1: ionic strength: 0.125 M; pH: 7.0; pressure: 1 atm; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQC IPAPsample_2anisotropicsample_conditions_1
2D HN(CO)CA IPAPsample_2anisotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N HSQC NOE 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

PALES, Zweckstetter M & Bax A - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC28797 BAG37199 BAG52620 BAG53672 BAJ21074
EMBL CAA73005 CAA73008
GB AAC79697 AAC79698 AAD12741 AAD12742 AAF07219
REF NP_001076446 NP_001092140 NP_001092141 NP_001162561 NP_001164963
SP P0C643 Q7LDG7 Q9QUG9
AlphaFold Q7LDG7 Q9QUG9 P0C643

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks