BMRB Entry 19315

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PDB ID: 2m9y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19315
MolProbity Validation Chart

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Title:
Solution Structure of the Catalytic Domain of HHARI
Deposition date:
2013-07-09
Original release date:
2013-11-11
Authors:
Mercier, Pascal; Spratt, Donald; Shaw, Gary
Citation:

Citation: Spratt, Donald; Mercier, Pascal; Gary, Shaw. "Structure of the HHARI catalytic domain shows glimpses of a HECT E3 ligase"  PLoS ONE 8, e74047-e74047 (2013).
PubMed: 24058416

Assembly members:

Assembly members:
entity_1, polymer, 74 residues, 8439.303 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKKCDDDSETSNWIAANTK ECPKCHVTIEKDGGSNHMVC RNQNCKAEFCWVCLGPWEPH GSAWYNCNRYNEDD

Data sets:
Data typeCount
13C chemical shifts222
15N chemical shifts87
1H chemical shifts420

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HHARI Catalytic Domain1
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, HHARI Catalytic Domain 74 residues - 8439.303 Da.

Residues 323-324 (N-terminal "GS") results from cloning and remains in the intact protein after TEV cleavage.

1   GLYSERLYSLYSCYSASPASPASPSERGLU
2   THRSERASNTRPILEALAALAASNTHRLYS
3   GLUCYSPROLYSCYSHISVALTHRILEGLU
4   LYSASPGLYGLYSERASNHISMETVALCYS
5   ARGASNGLNASNCYSLYSALAGLUPHECYS
6   TRPVALCYSLEUGLYPROTRPGLUPROHIS
7   GLYSERALATRPTYRASNCYSASNARGTYR
8   ASNGLUASPASP

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 1WD2 2M9Y 4KBL 4KC9
DBJ BAD92669 BAG35590
EMBL CAA08817 CAA10274 CAB45870 CAF98343 CAH65069
GB AAD28088 AAH38034 AAH51877 AAH57523 AAH57680
REF NP_001013126 NP_001025558 NP_001075183 NP_001089823 NP_001116952
SP A2VEA3 B1H1E4 Q32NS4 Q6NW85 Q6PFJ9
TPG DAA25726
AlphaFold A2VEA3 B1H1E4 Q32NS4 Q6NW85 Q6PFJ9

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