BMRB Entry 19274

Name: Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC
Published: 2014-12-22

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PDB ID: 2m8u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19274
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC

Deposition date:

2013-05-28

Original release date:

2014-12-22

Authors:

Liburd, Janine; Miller, Emily; Langelaan, David; Chitayat, Seth; Crawley, Scott; Cote, Graham; Smith, Steven

Citation:

Citation: Liburd, Janine; Miller, Emily; Langelaan, David; Chitayat, Seth; Crawley, Scott; Cote, Graham; Smith, Steven. "Solution Structure of the Dictyostelium discodieum Myosin Light Chain, MlcC" .

Assembly members:

Assembly members:
entity, polymer, 77 residues, 8918.021 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSHMNHINTKAQVIEAFKVF DRDGNGYVTVDYLRKVLNEL GDMMPADEIEEMIYEADPQN SGYVQYETFVGMLFLWD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	320
15N chemical shifts	81
1H chemical shifts	509

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dictyostelium discodieum Myosin Light Chain, MlcC	1

Entities:

Entity 1, Dictyostelium discodieum Myosin Light Chain, MlcC 77 residues - 8918.021 Da.

1

GLY

SER

HIS

MET

ASN

HIS

ILE

ASN

THR

LYS

2

ALA

GLN

VAL

ILE

GLU

ALA

PHE

LYS

VAL

PHE

3

ASP

ARG

ASP

GLY

ASN

GLY

TYR

VAL

THR

VAL

4

ASP

TYR

LEU

ARG

LYS

VAL

LEU

ASN

GLU

LEU

5

GLY

ASP

MET

PRO

ALA

ASP

GLU

ILE

GLU

6

GLU

MET

ILE

TYR

GLU

ALA

ASP

PRO

GLN

ASN

7

SER

GLY

TYR

VAL

GLN

TYR

GLU

THR

PHE

VAL

8

GLY

MET

LEU

PHE

LEU

TRP

ASP

Samples:

sample_1: entity 1 mM; HEPES 10 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 800 MHz

GB	EAL62662
REF	XP_636165