BMRB Entry 19268

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PDB ID: 2m8t
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19268
MolProbity Validation Chart

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Title:
SOLUTION NMR STRUCTURE OF THE V209M VARIANT OF THE HUMAN PRION PROTEIN (RESIDUES 90-231)
Deposition date:
2013-05-28
Original release date:
2013-09-10
Authors:
Mills, J.; Surewicz, K.; Surewicz, W.; Soennichsen, F.
Citation:

Citation: Kong, Qingzhong; Mills, Jeffrey; Kundu, Bishwajit; Li, Xinyi; Qing, Liuting; Surewicz, Krystyna; Cali, Ignazio; Huang, Shenghai; Zheng, Mengjie; Swietnicki, Wieslaw; Soennichsen, Frank; Gambetti, Pierluigi; Surewicz, Witold. "Thermodynamic Stabilization of the Folded Domain of Prion Protein Inhibits Prion Infection in Vivo"  Cell Rep. 4, 248-254 (2013).
PubMed: 23871665

Assembly members:

Assembly members:
MAJOR_PRION_PROTEIN, polymer, 146 residues, 16557.500 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: HUPRP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MAJOR_PRION_PROTEIN: GSDPGQGGGTHSQWNKPSKP KTNMKHMAGAAAAGAVVGGL GGYMLGSAMSRPIIHFGSDY EDRYYRENMHRYPNQVYYRP MDEYSNQNNFVHDCVNITIK QHTVTTTTKGENFTETDVKM MERMVEQMCITQYERESQAY YQRGSS

Data sets:
Data typeCount
13C chemical shifts425
15N chemical shifts160
1H chemical shifts918

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1MAJOR PRION PROTEIN1

Entities:

Entity 1, MAJOR PRION PROTEIN 146 residues - 16557.500 Da.

1   GLYSERASPPROGLYGLNGLYGLYGLYTHR
2   HISSERGLNTRPASNLYSPROSERLYSPRO
3   LYSTHRASNMETLYSHISMETALAGLYALA
4   ALAALAALAGLYALAVALVALGLYGLYLEU
5   GLYGLYTYRMETLEUGLYSERALAMETSER
6   ARGPROILEILEHISPHEGLYSERASPTYR
7   GLUASPARGTYRTYRARGGLUASNMETHIS
8   ARGTYRPROASNGLNVALTYRTYRARGPRO
9   METASPGLUTYRSERASNGLNASNASNPHE
10   VALHISASPCYSVALASNILETHRILELYS
11   GLNHISTHRVALTHRTHRTHRTHRLYSGLY
12   GLUASNPHETHRGLUTHRASPVALLYSMET
13   METGLUARGMETVALGLUGLNMETCYSILE
14   THRGLNTYRGLUARGGLUSERGLNALATYR
15   TYRGLNARGGLYSERSER

Related Database Links:

BMRB 15676 16743 16757 17714 17756 17757 17780 18426 18550 4379 4402 4434 4620 4641 4736
PDB 1E1G 1E1J 1E1P 1E1S 1E1U 1E1W 1FKC 1FO7 1HJM 1HJN 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3 2K1D 2KUN 2LEJ 2LFT 2LSB 2LV1 2M8T 2W9E 3HAF 3HAK 3HEQ 3HER 3HES 3HJ5 3HJX 4DGI 4KML 4N9O
DBJ BAF62360 BAG32276 BAG32278 BAG52189 BAG56832
EMBL CAA58442 CAG46836
GB AAA60182 AAA68632 AAB59442 AAB59443 AAC05365
REF NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP P04156 P61766 P61767 P61768
AlphaFold P04156 P61766 P61767 P61768

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