BMRB Entry 19235

Assembly:

Entities:

Entity 1, Aha1 chain 1 154 residues - 15135.344 Da.

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1.3 mM; TRIS-HCl 10 mM; NaCl 100 mM; DTT 5 mM; DSS 50 uM

sample_2: entity, [U-2H,13C,15N]; Ile 1-[13CH3]; Leu,Val-[13CH3], 0.7 mM; TRIS-HCl 10 mM; NaCl 100 mM; DTT 5 mM; DSS 50 uM

sample_3: entity, [U-5%-13C,100%-15N], 1.1 mM; TRIS-HCl 10 mM; NaCl 100 mM; DTT 5 mM; DSS 50 uM

sample_4: entity, [U-5%-13C,100%-15N], 1.1 mM; Pf1 phage 12.5 mg/mL; TRIS-HCl 10 mM; NaCl 100 mM; DTT 5 mM; DSS 50 uM

sample_5: entity, [U-5%-13C,100%-15N], 1.1 mM; PEG(C5E12) 4.0%; TRIS-HCl 10 mM; NaCl 100 mM; DTT 5 mM; DSS 50 uM

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

Software:

TOPSPIN v2.1, Bruker - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis

CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution

PSVS, Bhattacharya and Montelione - structure validation

NMR spectrometers:

• Bruker Avance 800 MHz
• Varian INOVA 600 MHz