BMRB Entry 19232

Name: NMR structure of a two-domain RNA-binding fragment of Nrd1
Published: 2014-05-12

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PDB ID: 2m88
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19232
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of a two-domain RNA-binding fragment of Nrd1

Deposition date:

2013-05-08

Original release date:

2014-05-12

Authors:

Bacikova, Veronika; Pasulka, Josef; Kubicek, Karel; Stefl, Richard

Citation:

Citation: Bacikova, Veronika; Pasulka, Josef; Kubicek, Karel; Stefl, Richard. "Structure and semi-sequence-specific RNA binding of Nrd1" .

Assembly members:

Assembly members:
entity, polymer, 185 residues, 20597.416 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: PDELNVSNNPHYRPKPVSYD STLPPDHIKVYSRTLFIGGV PLNMKEWDLANVLKPFAEVQ SVILNNSRKHAFVKVYSRHE AENVLQNFNKDGALPLRTRW GVGFGPRDCCDYQHGYSIIP MHRLTDADKKWSVSAQWGGT SGQPLVTGIVFEEPDIIVGE GVSSKAISQKMPTDSGRNGP RSGKP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	71
15N chemical shifts	127
1H chemical shifts	327

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	two-domain RNA-binding fragment of Nrd1	1

Entities:

Entity 1, two-domain RNA-binding fragment of Nrd1 185 residues - 20597.416 Da.

1

PRO

ASP

GLU

LEU

ASN

VAL

SER

ASN

PRO

2

HIS

TYR

ARG

PRO

LYS

PRO

VAL

SER

TYR

ASP

3

SER

THR

LEU

PRO

ASP

HIS

ILE

LYS

VAL

4

TYR

SER

ARG

THR

LEU

PHE

ILE

GLY

VAL

5

PRO

LEU

ASN

MET

LYS

GLU

TRP

ASP

LEU

ALA

6

ASN

VAL

LEU

LYS

PRO

PHE

ALA

GLU

VAL

GLN

7

SER

VAL

ILE

LEU

ASN

SER

ARG

LYS

HIS

8

ALA

PHE

VAL

LYS

VAL

TYR

SER

ARG

HIS

GLU

9

ALA

GLU

ASN

VAL

LEU

GLN

ASN

PHE

ASN

LYS

10

ASP

GLY

ALA

LEU

PRO

LEU

ARG

THR

ARG

TRP

11

GLY

VAL

GLY

PHE

GLY

PRO

ARG

ASP

CYS

12

ASP

TYR

GLN

HIS

GLY

TYR

SER

ILE

PRO

13

MET

HIS

ARG

LEU

THR

ASP

ALA

ASP

LYS

14

TRP

SER

VAL

SER

ALA

GLN

TRP

GLY

THR

15

SER

GLY

GLN

PRO

LEU

VAL

THR

GLY

ILE

VAL

16

PHE

GLU

PRO

ASP

ILE

VAL

GLY

GLU

17

GLY

VAL

SER

LYS

ALA

ILE

SER

GLN

LYS

18

MET

PRO

THR

ASP

SER

GLY

ARG

ASN

GLY

PRO

19

ARG

SER

GLY

LYS

PRO

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_2	isotropic	sample_conditions_2
3D HCACO	sample_2	isotropic	sample_conditions_2
4D HCCH methyl NOESY	sample_3	isotropic	sample_conditions_3
3D HCCCONH	sample_3	isotropic	sample_conditions_3
3D CCH TOCSY	sample_3	isotropic	sample_conditions_3

PDB	2M88
DBJ	GAA25857
EMBL	CAA65493 CAA96158 CAY82359
GB	AAC49568 AHY76857 AJP41095 AJT01532 AJT01902
REF	NP_014148
SP	P53617
TPG	DAA10308
AlphaFold	P53617

BMRB Entry 19232

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: