BMRB Entry 19228

Name: Human Pdx1 Homeodomain in the DNA-Bound State
Published: 2013-07-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19228

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Human Pdx1 Homeodomain in the DNA-Bound State

Deposition date:

2013-05-06

Original release date:

2013-07-29

Authors:

Showalter, Scott; Bastidas, Monique

Citation:

Citation: Bastidas, Monique; Showalter, Scott. "Thermodynamic and Structural Determinants of Differential Pdx1 Binding to Elements from the Insulin and IAPP Promoters." J. Mol. Biol. 425, 3360-3377 (2013).
PubMed: 23796517

Assembly members:

Assembly members:
Pdx1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-47b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pdx1: GPGNKRTRTAYTRAQLLELE KEFLFNKYISRPRRVELAVM LNLTERHIKIWFQNRRMKWK KEED

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_pdx1_dna

Data type	Count
13C chemical shifts	184
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Pdx1	1

Entities:

Entity 1, Pdx1 64 residues - Formula weight is not available

1

GLY

PRO

GLY

ASN

LYS

ARG

THR

ARG

THR

ALA

2

TYR

THR

ARG

ALA

GLN

LEU

GLU

LEU

GLU

3

LYS

GLU

PHE

LEU

PHE

ASN

LYS

TYR

ILE

SER

4

ARG

PRO

ARG

VAL

GLU

LEU

ALA

VAL

MET

5

LEU

ASN

LEU

THR

GLU

ARG

HIS

ILE

LYS

ILE

6

TRP

PHE

GLN

ASN

ARG

MET

LYS

TRP

LYS

7

LYS

GLU

ASP

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1

BMRB	19227
PDB	2H1K
DBJ	BAB32045 BAG74161
EMBL	CAA34746 CAA52389 CAA68169
GB	AAA18355 AAA74012 AAA88820 AAB18252 AAB29317
PIR	I65249
REF	NP_000200 NP_001074947 NP_001135456 NP_001165682 NP_001179065
SP	A1YF08 A1YG85 A2T756 P14837 P52945
TPG	DAA23869
AlphaFold	A1YF08 A1YG85 A2T756 P14837 P52945

BMRB Entry 19228

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: