BMRB Entry 19225

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PDB ID: 2m83
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19225
MolProbity Validation Chart

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Title:
Solution structure of the carbohydrate binding module of the muscle glycogen-targeting subunit of Protein Phosphatase-1
Deposition date:
2013-05-03
Original release date:
2014-05-12
Authors:
Koveal, Dorothy; Page, Rebecca; Peti, Wolfgang
Citation:

Citation: Koveal, Dorothy; Choy, Meng; Page, Rebecca; Peti, Wolfgang. "Molecular basis for Protein Phosphatase-1 regulation by the muscle glycogen-targeting subunit GM"  .

Assembly members:

Assembly members:
GM_CBM21, polymer, 139 residues, 15998.133 Da.

Natural source:

Natural source:   Common Name: Rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRP1B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GM_CBM21: GHMQTEEYVLSPLFDLPASK EDLMQQLQVQKAMLESTEYV PGSTSMKGIIRVLNISFEKL VYVRMSLDDWQTHYDILAEY VPNSCDGETDQFSFKISLVP PYQKDGSKVEFCIRYETSVG TFWSNNNGTNYTLVCQKKE

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts144
1H chemical shifts958

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1GM_CBM211

Entities:

Entity 1, GM_CBM21 139 residues - 15998.133 Da.

The first three residues (GHM) represent non-native residues that remain after cleavage of the N-terminal affinity tag

1   GLYHISMETGLNTHRGLUGLUTYRVALLEU
2   SERPROLEUPHEASPLEUPROALASERLYS
3   GLUASPLEUMETGLNGLNLEUGLNVALGLN
4   LYSALAMETLEUGLUSERTHRGLUTYRVAL
5   PROGLYSERTHRSERMETLYSGLYILEILE
6   ARGVALLEUASNILESERPHEGLULYSLEU
7   VALTYRVALARGMETSERLEUASPASPTRP
8   GLNTHRHISTYRASPILELEUALAGLUTYR
9   VALPROASNSERCYSASPGLYGLUTHRASP
10   GLNPHESERPHELYSILESERLEUVALPRO
11   PROTYRGLNLYSASPGLYSERLYSVALGLU
12   PHECYSILEARGTYRGLUTHRSERVALGLY
13   THRPHETRPSERASNASNASNGLYTHRASN
14   TYRTHRLEUVALCYSGLNLYSLYSGLU

Related Database Links:

PDB 2M83
GB AAA31462
REF NP_001075772
SP Q00756
AlphaFold Q00756

Download HSQC peak lists in one of the following formats:
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