BMRB Entry 19216

Name: Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2
Published: 2013-06-04

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PDB ID: 2m7x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19216
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2

Deposition date:

2013-05-02

Original release date:

2013-06-04

Authors:

Ullah, Asad; Kemp, Grant; Lee, Brian; Alves, Claudia; Young, Howard; Sykes, Brian; Fliegel, Larry

Citation:

Citation: Ullah, Asad; Kemp, Grant; Lee, Brian; Alves, Claudia; Young, Howard; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2." J. Biol. Chem. 288, 24609-24624 (2013).
PubMed: 23836910

Assembly members:

Assembly members:
sod2_TM_IV, polymer, 38 residues, 3073.673 Da.

Natural source:

Natural source: Common Name: Fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal-c2x

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sod2_TM_IV: GSKKKLFPQINFLGSLLIAG CITSTDPVLSALIVGKKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	35
1H chemical shifts	176

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sod2_TM_IV	1

Entities:

Entity 1, sod2_TM_IV 38 residues - 3073.673 Da.

1

GLY

SER

LYS

LEU

PHE

PRO

GLN

ILE

2

ASN

PHE

LEU

GLY

SER

LEU

ILE

ALA

GLY

3

CYS

ILE

THR

SER

THR

ASP

PRO

VAL

LEU

SER

4

ALA

LEU

ILE

VAL

GLY

LYS

Samples:

sample_1: sod2_TM_IV, [U-99% 15N], 0.2 – 0.7 mM; CDCl3 50 v/v; 2-propanol 50 v/v

sample_conditions_1: pressure: 1 atm; temperature: 303.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY-HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY-HSQC	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ, Johnson, One Moon Scientific - data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Varian INOVA 500 MHz