BMRB Entry 19195

Name: alfa-actinin from parasite Entamoeba histolytica
Published: 2013-06-04

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PDB ID: 2m7l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19195
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

alfa-actinin from parasite Entamoeba histolytica

Deposition date:

2013-04-26

Original release date:

2013-06-04

Authors:

Persson, Cecilia; Mayzel, Maxim; Karlsson, Goran; Backman, Lars

Citation:

Citation: Persson, Cecilia; Mayzel, Maxim; Karlsson, Goran; Backman, Lars. "alfa-actinin from parasite Entamoeba histolytica" .

Assembly members:

Assembly members:
entity, polymer, 144 residues, 15924.354 Da.

Natural source:

Natural source: Common Name: Entamoeba Histolytica Taxonomy ID: 5759 Superkingdom: Eukaryota Kingdom: not available Genus/species: Entamoeba Histolytica

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSSSGVTAEQMQEFKQSFDA FDGNHDGILDKLEFRSCLSS MGLIDIDFTGGEDAQYDAIY NNVTKGENGVSFDNYVQYMK EKNDENPSPEQLNEIFSTIA AGKDSITETDMQKAGMSAEQ IEYVKANLPQKGDGYDYAAW VKTN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	537
15N chemical shifts	136
1H chemical shifts	869

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alfa-actinin from parasite Entamoeba histolytica	1

Entities:

Entity 1, alfa-actinin from parasite Entamoeba histolytica 144 residues - 15924.354 Da.

1

GLY

SER

GLY

VAL

THR

ALA

GLU

GLN

2

MET

GLN

GLU

PHE

LYS

GLN

SER

PHE

ASP

ALA

3

PHE

ASP

GLY

ASN

HIS

ASP

GLY

ILE

LEU

ASP

4

LYS

LEU

GLU

PHE

ARG

SER

CYS

LEU

SER

5

MET

GLY

LEU

ILE

ASP

ILE

ASP

PHE

THR

GLY

6

GLY

GLU

ASP

ALA

GLN

TYR

ASP

ALA

ILE

TYR

7

ASN

VAL

THR

LYS

GLY

GLU

ASN

GLY

VAL

8

SER

PHE

ASP

ASN

TYR

VAL

GLN

TYR

MET

LYS

9

GLU

LYS

ASN

ASP

GLU

ASN

PRO

SER

PRO

GLU

10

GLN

LEU

ASN

GLU

ILE

PHE

SER

THR

ILE

ALA

11

ALA

GLY

LYS

ASP

SER

ILE

THR

GLU

THR

ASP

12

MET

GLN

LYS

ALA

GLY

MET

SER

ALA

GLU

GLN

13

ILE

GLU

TYR

VAL

LYS

ALA

ASN

LEU

PRO

GLN

14

LYS

GLY

ASP

GLY

TYR

ASP

TYR

ALA

TRP

15

VAL

LYS

THR

ASN

Samples:

sample_1: The protein, [U-100% 13C; U-100% 15N], 1.5 mM; sodium phosphate 25 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS v2.2, CCPN - assignment and data analysis

DANGLE v1.1 -

CYANA v2.2, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 900 MHz

PDB	2M7L
GB	EAL47897 EKE40069 EMD43687 EMH76125 EMS12580
REF	XP_008857596 XP_653283