BMRB Entry 19163

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PDB ID: 2m6y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19163
MolProbity Validation Chart

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Title:
The solution structure of the J-domain of human DnaJA1
Deposition date:
2013-04-14
Original release date:
2013-06-24
Authors:
Stark, Jaime; Mehla, Kamiya; Chaika, Nina; Acton, Thomas; Xiao, Rong; Singh, Pankaj; Montelione, Gaetano; Powers, Robert
Citation:

Citation: Stark, Jaime; Mehla, Kamiya; Chaika, Nina; Acton, Thomas; Xiao, Rong; Singh, Pankaj; Montelione, Gaetano; Powers, Robert. "Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer."  Biochemistry 53, 1360-1372 (2014).
PubMed: 24512202

Assembly members:

Assembly members:
J-domain_of_DnaJA1, polymer, 77 residues, 9095.388 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
J-domain_of_DnaJA1: MGHHHHHHSHMVKETTYYDV LGVKPNATQEELKKAYRKLA LKYHPDKNPNEGEKFKQISQ AYEVLSDAKKRELYDKG

Data sets:
Data typeCount
13C chemical shifts277
15N chemical shifts63
1H chemical shifts430

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1J-domain of human DnaJA11

Entities:

Entity 1, J-domain of human DnaJA1 77 residues - 9095.388 Da.

Residues 1-10 represent a non-native affinity tag

1   METGLYHISHISHISHISHISHISSERHIS
2   METVALLYSGLUTHRTHRTYRTYRASPVAL
3   LEUGLYVALLYSPROASNALATHRGLNGLU
4   GLULEULYSLYSALATYRARGLYSLEUALA
5   LEULYSTYRHISPROASPLYSASNPROASN
6   GLUGLYGLULYSPHELYSGLNILESERGLN
7   ALATYRGLUVALLEUSERASPALALYSLYS
8   ARGGLULEUTYRASPLYSGLY

Related Database Links:

BMRB 18190
PDB 2LO1 2M6Y
DBJ BAA02656 BAC38744 BAC82111 BAD82815 BAE26788
EMBL CAI29674
GB AAA98855 AAC37517 AAC78597 AAH08182 AAH57876
PIR S34632
REF NP_001015637 NP_001127102 NP_001158143 NP_001158144 NP_001231092
SP P31689 P63036 P63037 Q5E954 Q5NVI9
TPG DAA12974 DAA26671
AlphaFold P31689 P63036 P63037 Q5E954 Q5NVI9

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