BMRB Entry 19077

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PDB ID: 2muu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19077
MolProbity Validation Chart

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Title:
1H, 13C and 15N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domain of the human deubiquitinase USP28
Deposition date:
2013-03-06
Original release date:
2014-02-13
Authors:
Wen, Yi; Cui, Rong; Zhang, Huaqun; Zhang, Naixia
Citation:

Citation: Wen, Yi; Cui, Rong; Zhang, Huaqun; Zhang, Naixia. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domains of the human deubiquitinase Usp28."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23754699

Assembly members:

Assembly members:
USP28, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGBTNH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
USP28: GSMTAELQQDDAAGAADGHG SSCQMLLNQLREITGIQDPS FLHEALKASNGDITQAVSLL TDERVKEPSQDTVATEPSEV EGSAANKEVLAKVIDLTHDN KDDLQAAIALSLLESPKIQA DGVDKLAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts111
1H chemical shifts764

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1USP28 monomer1

Entities:

Entity 1, USP28 monomer 137 residues - Formula weight is not available

The first two residues GS and the last fifteen residues VDKLAAALEHHHHHH are cloning artifacts.

1   GLYSERMETTHRALAGLULEUGLNGLNASP
2   ASPALAALAGLYALAALAASPGLYHISGLY
3   SERSERCYSGLNMETLEULEUASNGLNLEU
4   ARGGLUILETHRGLYILEGLNASPPROSER
5   PHELEUHISGLUALALEULYSALASERASN
6   GLYASPILETHRGLNALAVALSERLEULEU
7   THRASPGLUARGVALLYSGLUPROSERGLN
8   ASPTHRVALALATHRGLUPROSERGLUVAL
9   GLUGLYSERALAALAASNLYSGLUVALLEU
10   ALALYSVALILEASPLEUTHRHISASPASN
11   LYSASPASPLEUGLNALAALAILEALALEU
12   SERLEULEUGLUSERPROLYSILEGLNALA
13   ASPGLYVALASPLYSLEUALAALAALALEU
14   GLUHISHISHISHISHISHIS

Related Database Links:

BMRB 18560
PDB 2LVA
DBJ BAG65214
GB AAH65928 AAK58565 ACA06098 ACA06099 AIC60511
REF NP_065937 XP_001085291 XP_002799842 XP_003253212 XP_004052203
SP Q96RU2
AlphaFold Q96RU2

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CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks